Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 110, Issue 2, pp 261–273 | Cite as

Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications

  • Guanze Liu
  • Shuangxia Jin
  • Xuyan Liu
  • Jiafu Tan
  • Xiyan Yang
  • Xianlong Zhang
Original Paper

Abstract

The D-class cyclin genes play key roles in controlling the cell cycle in plant development. To evaluate the effects of D-type cyclins on plant architecture, ectopic expression of the ArabidopsiscyclinD2;1 (Arath;CYCD2;1) gene driven by the CaMV35S promoter was investigated in cotton via Agrobacterium-mediated transformation. Northern blot showed the cyclinD2;1 gene was highly expressed in transgenic cotton plants. Phenotype investigation showed that overexpression of Arath;CYCD2;1 led to obvious leaf architecture change: the leaf epidermis of transgenic plants consist of more small cells compared to the wild-type. Mesophyll cells in the inner layers of Arath;CYCD2;1 plants were organized more loosely than those in the inner layers of wild-type plants. Moreover, transgenic plants had darker leaves, more chlorophyll, and a higher rate of photosynthesis than wild-type plants in the field. Tissue cultures indicated that the overexpression of Arath;CYCD2;1 promoted callus formation in the absence of exogenous auxin, but inhibited cell differentiation. The qRT-PCR revealed that several cell cycle–associated genes, particularly the transcript levels of GhRBR and GhCYCD3;1 were regulated by the Arath;CYCD2;1 insertion. The results implied that the cotton plant architecture or cell culture characters could be regulated by ectopic expression of the ArabidopsiscyclinD2;1 gene.

Keywords

D-type cyclins Arath;CYCD2;1 Leaf rolling Plant architecture Transgenic cotton 

Abbreviations

CaMV

Cauliflower mosaic virus

CDK

Cyclin-dependent kinase

ICK

Inhibitor of cyclin-dependent kinase

RBR

Retinoblastoma-related protein

NPA

N-1-naphthylphthalamic acid

MCM

Minichromosomal maintenance

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Guanze Liu
    • 1
  • Shuangxia Jin
    • 1
  • Xuyan Liu
    • 1
  • Jiafu Tan
    • 1
  • Xiyan Yang
    • 1
  • Xianlong Zhang
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanPeople’s Republic of China

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