Overexpression of Arabidopsis cyclin D2;1 in cotton results in leaf curling and other plant architectural modifications
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The D-class cyclin genes play key roles in controlling the cell cycle in plant development. To evaluate the effects of D-type cyclins on plant architecture, ectopic expression of the Arabidopsis cyclinD2;1 (Arath;CYCD2;1) gene driven by the CaMV35S promoter was investigated in cotton via Agrobacterium-mediated transformation. Northern blot showed the cyclinD2;1 gene was highly expressed in transgenic cotton plants. Phenotype investigation showed that overexpression of Arath;CYCD2;1 led to obvious leaf architecture change: the leaf epidermis of transgenic plants consist of more small cells compared to the wild-type. Mesophyll cells in the inner layers of Arath;CYCD2;1 plants were organized more loosely than those in the inner layers of wild-type plants. Moreover, transgenic plants had darker leaves, more chlorophyll, and a higher rate of photosynthesis than wild-type plants in the field. Tissue cultures indicated that the overexpression of Arath;CYCD2;1 promoted callus formation in the absence of exogenous auxin, but inhibited cell differentiation. The qRT-PCR revealed that several cell cycle–associated genes, particularly the transcript levels of GhRBR and GhCYCD3;1 were regulated by the Arath;CYCD2;1 insertion. The results implied that the cotton plant architecture or cell culture characters could be regulated by ectopic expression of the Arabidopsis cyclinD2;1 gene.
KeywordsD-type cyclins Arath;CYCD2;1 Leaf rolling Plant architecture Transgenic cotton
Cauliflower mosaic virus
Inhibitor of cyclin-dependent kinase
The authors thank Dr. Y. M. Zhou for his generosity in providing the CycD2 cDNA vector and Dr. J. L. Yao for her help with histological observation. This work was supported by the National Natural Science Foundation of China (No. 30810103911).
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