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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 110, Issue 2, pp 189–200 | Cite as

A newly isolated Na+/H+ antiporter gene, DmNHX1, confers salt tolerance when expressed transiently in Nicotiana benthamiana or stably in Arabidopsis thaliana

  • Hui Zhang
  • Yaxin Liu
  • Yuan Xu
  • Sean Chapman
  • Andrew J. Love
  • Tao XiaEmail author
Original Paper

Abstract

Sodium/hydrogen transporters transfer ions across membranes and thus play an important role in pH and electrolyte homeostasis. To further understand the mechanism and function of the plant vacuolar Na+/H+, a new Na+/H+ antiporter, named DmNHX1, was isolated from chrysanthemum (Dendranthema morifolium) and characterized. The total length of DmNHX1 is 1,897 bp, with an open reading frame of 1,653 bp. Functional complementation studies in Saccharomyces cerevisiae strains with mutated endogenous NHX genes showed that the encoded protein, DmNHX1, has a broad cation specificity (Na+, K+, Li+) and confered hygromycin B tolerance. Quantitative RT-PCR revealed that the transcription level of DmNHX1 was induced by salt stress in all studied tissues, especially expressed highest in the leaves. Agrobacterium-mediated transient expression of DmNHX1 in Nicotiana benthamiana enhanced the salt tolerance of leaf discs, as indicated by reduced bleaching and increased chlorophyll content when compared to that of controls. In addition, overexpressing DmNHX1 resulted in enhanced salinity tolerance in transgenic Arabidopsis thaliana. Our studies suggest that DmNHX1 plays the crucial role in salt transport of chrysanthemum and could be applied for the improvement of salt tolerance.

Keywords

Chrysanthemum Na+/H+ antiporter Transient expression Salt tolerance 

Abbreviations

NHX1

Na+/H+ exchanger

RT-PCR

Reverse-transcription PCR

RACE

Rapid amplification of cDNA ends

QPCR

Quantitative real time PCR

Notes

Acknowledgments

We thank The James Hutton Institute for excellent glasshouse supplement; and the academic visit opportunity from China Scholarship Council. This work was supported by the grant of Innovation Fund of Shanghai Municipal Education Commission.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Hui Zhang
    • 1
  • Yaxin Liu
    • 1
  • Yuan Xu
    • 1
  • Sean Chapman
    • 2
  • Andrew J. Love
    • 2
  • Tao Xia
    • 1
    Email author
  1. 1.College of Life ScienceEast China Normal UniversityShanghaiChina
  2. 2.The James Hutton InstituteInvergowrie, DundeeUK

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