In vitro propagation and characterization of phenolic content along with antioxidant and antimicrobial activities of Cichorium pumilum Jacq.
- 650 Downloads
Cichorium pumilum, a member of Asteraceae, is widely used as a traditional medicinal herb. An efficient protocol for callus formation and whole plant propagation has been developed. Callus cultures were induced from leaf explants on Murashige and Skoog (MS) medium supplemented with 1.5 mg l−1 6-Benzyladenine (BA) and 0.5 mg l−1 Naphthalene acetic acid (NAA). Maximum numbers of shoots were obtained from calli transferred to shoot regeneration medium containing MS basal medium with 1.5 mg l−1 BA or Kinetin (Kin). The shoots were effectively rooted on MS medium supplemented with different concentrations of Indole-3-butyric acid. In the present study, the antibacterial activity of C. pumilum extracts was assayed in vitro by agar disc diffusion and agar well diffusion methods against 10 different bacterial species. The results showed effect on the growth of 50 and 70% of the tested bacterial species using methanol and ethanol extracts respectively. Klebsiella pneumoniae was susceptible to the ethanolic and methanolic extracts of wild plants and in vitro tissues, whereas Enterococcus faecalis was resistant to all the extracts. This study verified that the methanol extracts have strong antioxidant activities with high levels of phenolic compounds. The antioxidant activity and total phenol content of callus cultures and in vitro plantlets were lower than those of the wild plants. The results obtained confirm the therapeutic potency of Cichorium used in the traditional medicine, in addition, the efficient in vitro production system developed in this study provide sterile and consistent tissues for the investigation of phytochemical and pharmacological effects and germplasm conservation of C. pumilum.
KeywordsCichorium pumilum In vitro Phenolic content Antioxidant Antimicrobial
The authors would like to thank the deanship of research at Yarmouk University for Funding this Study, Project #22/2009.
- Abu Irmaileh B (1980) Weeds of Jordan (Weeds of crop fields). University of Jordan press, AmmanGoogle Scholar
- Abu-Shanab B, Adwan G, Abu Safiya D, Jarrar N, Adwan K (2004) Antibacterial activities of some plant extracts utilized in popular medicine in Palestine. Turk J Biol 28:99–102Google Scholar
- Akroum S, Satta D, Lalaoui K (2009) Antimicrobial, antioxidant, cytotoxic activities and phytochemical screening of some algerian plants. Eur J Sci Res 31(2):289–295Google Scholar
- Cowan M (1999) Plant products as antimicrobical agents. CMR 12:564–582Google Scholar
- Hassan H, Rahman S, Deeba F, Mahmud S (2009) Antimicrobial activity of some plant extracts having hepatoprotective effects. J Med Plants Res 3(1):20–23Google Scholar
- Kisiel W, Michalska K (2003) Root constituents of Cichorium pumilum and rearrangement of some lactucin-like guaianolides. Z Naturforsch 58:789–792Google Scholar
- Malarz J, Stojakowska A, Kisiel W (2002) Sesquiterpene lactones in a hairy root culture of Cichorium intybus. Z Naturforsch 57:994–997Google Scholar
- Molchan O, Romashko S, Yuri V (2012) l-tryptophan decarboxylase activity and tryptamine accumulation in callus cultures of Vinca minor L. Plant Cell Tissue Organ Cult. doi: 10.1007/s11240-011-0060
- Nandagopal S, Kumari B (2007) Effectiveness of auxin induced in vitro root culture in chicory. J Cent Eur Agric 8:73–80Google Scholar
- Pinto M, Kwon Y, Apostolidis E, Lajolo F, Genovese M, Shetty K (2008) Functionality of bioactive compounds in Brazilian strawberry (Fragaria × ananassa Duch.) cultivars: evaluation of hyperglycemia and hypertension potential using in vitro models. J Agric Food Chem 56:4386–4392CrossRefGoogle Scholar
- Rehman R, Israr M, Srivastava P, Bansal K, Abdin M (2003) In vitro regeneration of witloof chicory (Cichorium intybus L.) from leaf explants and accumulation of esculin. In Vitro Cell Dev Biol 39:142–146Google Scholar
- Velayutham P, Ranjithakumari B, Baskaran P (2006) An efficient in vitro plant regeneration system for Cichorium intybus L.–an important medicinal plant. J Agricult Tech 2:287–298Google Scholar