Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis
Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l−1 and PMEDAP at concentrations 50 and 12.5 mg l−1 significantly (P < 0.05) reduced CaMV concentration in plants within 42–63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12.5, 25 and 50 mg l−1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0.05) already at 12.5 mg l−1 with both compounds.
KeywordsCaulimovirus dsDNA Chemotherapy Pararetrovirus Phytotoxicity Virus eradication
Acyclic nucleoside phosphonate
Cauliflower mosaic virus
Murashige and Skoog medium
This project was funded by grants nos. 522/09/0707 from the Grant Agency of the Czech Republic and AV0Z50510513 from the Academy of Sciences of the Czech Republic, as well as by Research Project OZ40550506 of the Institute of Organic Chemistry and Biochemistry. The authors thank Dr. I. Dostálková, Ph.D., Faculty of Science, University of South Bohemia, for consultations on statistical processing of the data.