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Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis

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Antiviral effects of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP, and ribavirin on double-stranded DNA Cauliflower mosaic virus (CaMV) were evaluated in Brassica pekinensis plants grown in vitro on liquid medium. A double-antibody sandwich ELISA was used for relative quantification of viral protein and PCR for detection of CaMV nucleic acid in plants. Ribavirin and PMEA had no significant antiviral effect. (S)-HPMPC at concentration 50 mg l−1 and PMEDAP at concentrations 50 and 12.5 mg l−1 significantly (P < 0.05) reduced CaMV concentration in plants within 42–63 days to levels detectable neither by ELISA nor by PCR. A phytotoxicity experiment resulted in progressive yellowing of leaves and dwarfing in plants cultured 42 days on media with concentrations 12.5, 25 and 50 mg l−1 of (S)-HPMPC and PMEDAP. Reduction in fresh and dry weights of plants was significant (P < 0.05) already at 12.5 mg l−1 with both compounds.

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Acyclic nucleoside phosphonate


Cauliflower mosaic virus


Murashige and Skoog medium










  1. Acquadro A, Papanice MA, Lanteri S, Bottalico G, Portis E, Campanale A, Finetti-Sialer MM, Mascia T, Sumerano P, Gallitelli D (2010) Production and fingerprinting of virus-free clones in a reflowering globe artichoke. Plant Cell Tissue Organ Cult 100:329–337

  2. Cheong EJ, Mock R, Li R (2012) Elimination of five viruses from sugarcane using in vitro culture of axillary buds and apical meristems. Plant Cell Tissue Organ Cult. doi:10.1007/s11240-011-0108-3

  3. De Clercq E (2011) The clinical potential of the acyclic (and cyclic) nucleotide phosphonates. The magic of the phosphonate bond. Biochem Pharmacol 82:99–109

  4. Hansen AJ (1989) Antiviral chemicals for plant disease control. Crit Rev Plant Sci 8:45–88

  5. Hanzliková-Vašková D, Špak J, Petrzik K (2006) Variability in sequence of Strawberry vein banding virus. Biol Plant 50:660–666

  6. Helliot B, Panis B, Frison E, De Clercq E, Swennen R, Lepoivre P, Neyts J (2003) The acyclic nucleoside phosphonate analogues, adefovir, tenofovir and PMEDAP, efficiently eliminate banana streak virus from banana (Musa ssp.). Antiviral Res 59:121–126

  7. Holý A, Rosenberg I, Dvořáková H (1989) Synthesis of N-(2-phosphonylmethoxy-ethyl) derivatives of heterocyclic bases. Collect Czech Chem Commun 54:2190–2210

  8. Murashige TF, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497

  9. Ramgareeb S, Snyman SJ, van Antwerpen T, Rutherford RS (2010) Elimination of virus and rapid propagation of disease-free sugarcane (Saccharum spp. cultivar NCo376) using apical meristem culture. Plant Cell Tissue Organ Cult 100:175–181

  10. Rommens CMT, Kneppers TJA, Haring MA, Nijkamp HJJ, Hille J (1991) A transposon tagging strategy with/Ac/on plant cell level in heterologous plant species. Plant Sci 74:99–106

  11. Špak J (1989a) Characterisation of Czechoslovak isolates of Cauliflower mosaic virus from brassica crops. Ochrana rostlin 25:177–181

  12. Špak J (1989b) Purification and serodiagnosis of Cauliflower mosaic virus. Ochrana rostlin 25:241–247

  13. Špak J, Holý A, Pavingerová D, Votruba I, Špaková V, Petrzik K (2010) New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses. Antiviral Res 88:296–303

  14. Špak J, Votruba I, Pavingerová D, Holý A, Špaková V, Petrzik K (2011) Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in B. pekinensis plants. Antiviral Res 92:378–381

  15. Tan R, Wang L, Hong N, Wang G (2010) Enhanced efficiency of virus eradication following thermotherapy of shoot-tip cultures of pear. Plant Cell Tissue Organ Cult 101:229–235

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This project was funded by grants nos. 522/09/0707 from the Grant Agency of the Czech Republic and AV0Z50510513 from the Academy of Sciences of the Czech Republic, as well as by Research Project OZ40550506 of the Institute of Organic Chemistry and Biochemistry. The authors thank Dr. I. Dostálková, Ph.D., Faculty of Science, University of South Bohemia, for consultations on statistical processing of the data.

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Correspondence to Josef Špak.

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Špak, J., Votruba, I., Pavingerová, D. et al. Antiviral activity of acyclic nucleoside phosphonates PMEA, (S)-HPMPC, PMEDAP and ribavirin against Cauliflower mosaic virus in Brassica pekinensis . Plant Cell Tiss Organ Cult 110, 63–68 (2012). https://doi.org/10.1007/s11240-012-0130-0

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  • Caulimovirus
  • dsDNA
  • Chemotherapy
  • Pararetrovirus
  • Phytotoxicity
  • Virus eradication