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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 109, Issue 3, pp 501–508 | Cite as

Somatic embryogenesis and plant regeneration in açaí palm (Euterpe oleracea)

  • Jonny Everson Scherwinski-Pereira
  • Rodrigo da Silva Guedes
  • Ricardo Alexandre da Silva
  • Paulo César Poeta FerminoJr.
  • Zanderluce Gomes Luis
  • Elínea de Oliveira Freitas
Original Paper

Abstract

An efficient and simple system for inducing somatic embryogenesis and regenerating plantlets from immature zygotic embryos of açaí palm (Euterpe oleracea) has been developed. Embryogenic calli (ECs) were induced from immature zygotic embryos of açaí palm on Murashige and Skoog (MS) modified medium with 2,4-dichlorophenoxyacetic acid and picloram. Embryogenic frequency was dependent on auxin type and concentration. The optimal concentration of picloram for the high-frequency induction of embryogenic calli (72%) was 225 μM. ECs were then subcultured on a differentiation and maturation medium composed of MS modified medium with 2-isopentenyladenine and naphthaleneacetic acid with subcultures at 4-week intervals. SEs were converted to plants on MS modified medium with half-strength macro- and micronutrients, 20 g l−1 sucrose, and 2.5 g l−1 activated charcoal and gelled with 2.5 g l−1 Phytagel. Detailed morpho anatomical changes during the different stages of somatic embryogenesis were characterized. The development of SEs was asynchronous, and ontogenic studies confirmed that the initial cell divisions occur in the epidermal and subepidermal regions of the zygotic embryos. Broad base attachment of SEs to the epidermis indicates the presence of a suspensor.

Keywords

Arecaceae Acai berry palm Euterp palm Morphogenesis Embryo origin Ontogenesis Somatic embryos 

Abbreviations

EC

Embryogenic calli

MS

Murashige and Skoog medium (1962)

2iP

2-Isopentenyladenine

2,4-D

2,4-Dichlorophenoxyacetic acid

NAA

Naphthaleneacetic acid

SEs

Somatic embryos

Notes

Acknowledgments

The authors thank the Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, Brasília, Brazil, for financial support and fellowships. We wish to thank Simone A. Maciel for technical assistance.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jonny Everson Scherwinski-Pereira
    • 1
  • Rodrigo da Silva Guedes
    • 2
  • Ricardo Alexandre da Silva
    • 3
  • Paulo César Poeta FerminoJr.
    • 4
  • Zanderluce Gomes Luis
    • 5
  • Elínea de Oliveira Freitas
    • 5
  1. 1.Embrapa Genetic Resources and BiotechnologyBrasíliaBrazil
  2. 2.IDARON—Agência de Defesa Sanitária Agrossilvopastoril do Estado de RondôniaPorto VelhoBrazil
  3. 3.Federal University of Rio de JaneiroRio de JaneiroBrazil
  4. 4.Federal University of AcreRio BrancoBrazil
  5. 5.University of BrasíliaBrasíliaBrazil

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