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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 107, Issue 3, pp 451–459 | Cite as

In vitro induction of autotetraploids from diploid yellow passion fruit mediated by colchicine and oryzalin

  • M. M. Rêgo
  • E. R. Rêgo
  • C. H. Bruckner
  • F. L. Finger
  • W. C. Otoni
Original Paper

Abstract

In vitro chromosome doubling from hypocotyl segments of yellow passion fruit (Passiflora edulis Sims.) was carried out in the presence of either colchicine (0, 25, 250 and 1,250 μM) or oryzalin (0, 5, 15 and 30 μM). Murashige and Skoog (in Physiol Plant 15:473–497, 1962)(MS)-based regeneration medium containing 250 or 1,250 μM colchicine markedly affected explant development leading to browning and death of the hypocotyl segments. Oryzalin has similar effect to colchicine in inducing polyploidy. In vitro regenerated autotetraploid plants induced by 25 μM colchicine or 15 μM oryzalin were further acclimatized and cultivated in hydroponics system in greenhouse. Autotetraploids plants were more vigorous than the control diploids. The chromosome number of diploid plants was 2n = 2x = 18, whereas that found on autotetraploid plants were 2n = 4x = 36. The stomata sizes of the autotetraploids were significantly larger than those on the diploid counterparts, while the frequency of stomata was significantly reduced. Similarly, the chloroplast number of guard cells of autotetraploid plants increased significantly. Two albino plants (4%) were generated in medium with 25 μM colchicine, indicating phytotoxic effects. These plants are being grown to full maturity in order to test their potential to use in a breeding program.

Keywords

Polyploidy Passiflora Tissue culture Breeding 

Abbreviations

MS

Murashige and Skoog (1962)

B5

Gamborg medium (1968)

BA

6-Benzyladenine

Kin

Kinetin

IAA

Indole-3-acetic acid

Notes

Acknowledgments

The authors thank CNPq and CAPES for financial support this investigation.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • M. M. Rêgo
    • 1
  • E. R. Rêgo
    • 1
  • C. H. Bruckner
    • 2
  • F. L. Finger
    • 2
  • W. C. Otoni
    • 3
  1. 1.Universidade Federal da Paraíba, Centro de Ciências AgráriasAreiaBrazil
  2. 2.Departamento de FitotecniaUniversidade Federal de ViçosaViçosaBrazil
  3. 3.Departamento de Biologia VegetalUniversidade Federal de ViçosaViçosaBrazil

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