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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 106, Issue 3, pp 409–418 | Cite as

Somatic embryogenesis in the medicinal legume Desmodium motorium (Houtt.) Merr.

  • B. Chitra Devi
  • V. Narmathabai
Original Paper

Abstract

An efficient protocol was established for regeneration of Desmodium motorium via somatic embryogenesis. Embryogenic calli were induced from cotyledon segments (6 mm, 16 days old) lacking embryo axis, excised from seedlings grown in vitro on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA) (2.9 μM) in combination with 6-benzyladenine (BA) (4.44 and 8.88 μM). Differentiation of embryogenic calli into globular and heart-shaped somatic embryos was achieved on transfer to hormone-free MS medium. When incubated for 4 days on MS medium supplemented with BA (8.88 μM), 95% of the globular and heart-shaped somatic embryos matured into torpedo and cotyledonary stages with minimum (10%) abnormalities. Modified MS basal medium without hormones and containing half-strength macronutrients and 0.88 M sucrose was suitable for germination of mature somatic embryos. Regenerated plantlets were successfully transferred to earthen pots with survival rate of 50%. Secondary embryogenesis was observed when pre-existing somatic embryos at globular and heart-shaped stages were cultured on MS medium supplemented with various concentrations of BA, adenine sulphate (AdS) and abscisic acid (ABA) individually.

Keywords

Somatic embryogenesis Fabaceae Cotyledon explants Secondary embryogenesis 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

ABA

Abscisic acid

AdS

Adenine sulphate

BA

6-Benzyladenine

IAA

Indole-3-acetic acid

MS

Murashige and Skoog (1962) medium

NAA

α-Naphthalene acetic acid

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© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.Department of BiotechnologyKarpagam UniversityCoimbatoreIndia
  2. 2.Department of BotanyBharathiar UniversityCoimbatoreIndia

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