Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress

  • Honghong Fan
  • Tingchun Li
  • Lei Guan
  • Zhengpeng Li
  • Ning Guo
  • Yongping Cai
  • Yi LinEmail author
Original Paper


Nitric oxide (NO) is an important biological messenger in plants, which has been implicated in response to abiotic stress. To study the effects of exogenous NO on drought menace, the tube seedlings of Dendrobium huoshanense were selected and treated with 10% polyethylene glycol (PEG-6000) to simulate drought stress. After application of sodium nitroprusside (SNP), the relative water content (RWC) and antioxidant enzyme activities were determined. As a result, plant treated with 50 μmol L−1 of SNP maintained high level of RWC and lower content of malondialdehyde (MDA). Furthermore, the antioxidant enzyme activities were obviously enhanced. However, the higher concentration of SNP (100 μmol L−1) enhanced the effects of drought stress for plant. For further analysis of the response mechanism to exogenous NO, the methylation-sensitive amplified polymorphism (MSAP) technique was used to investigate the changes of DNA methylation. When the seedlings of Dendrobium huoshanense were treated with 50 μmol L−1 SNP containing 10% PEG-6000, levels of global DNA methylation of Dendrobium huoshanense were decreased. Nevertheless, the demethylation rate of methylated sites increased, accounting for 12.5% of total methylation sites. These results implied that some expressed genes were involved in the response process to drought stress triggered by NO in Dendrobium huoshanense.


Dendrobium huoshanense Exogenous nitric oxide Methylation sensitive amplified polymorphism (MSAP) Drought stress DNA methylation Sodium nitroprusside (SNP) 



Nitric oxide


Sodium nitroprusside


Polyethylene glycol-6000


Methylation-sensitive amplified polymorphism


Murashige and Skoog


Superoxide dismutase











The present investigation is financially supported by Anhui Provincial Natural Science Foundation for Distinguished Young Scholars (10040606Q22), the University Excellent Young Talents Foundation of Anhui Province (2011SQRL053) and Anhui agricultural University Young Talents Foundation (yj2008-27).


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Honghong Fan
    • 1
  • Tingchun Li
    • 1
    • 2
  • Lei Guan
    • 1
  • Zhengpeng Li
    • 1
  • Ning Guo
    • 1
  • Yongping Cai
    • 1
  • Yi Lin
    • 1
    Email author
  1. 1.Department of Life SciencesAnhui Agricultural UniversityHefeiPeople’s Republic of China
  2. 2.Anhui Academy of Agricultural SciencesHefeiPeople’s Republic of China

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