Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense grown under drought stress
- 797 Downloads
Nitric oxide (NO) is an important biological messenger in plants, which has been implicated in response to abiotic stress. To study the effects of exogenous NO on drought menace, the tube seedlings of Dendrobium huoshanense were selected and treated with 10% polyethylene glycol (PEG-6000) to simulate drought stress. After application of sodium nitroprusside (SNP), the relative water content (RWC) and antioxidant enzyme activities were determined. As a result, plant treated with 50 μmol L−1 of SNP maintained high level of RWC and lower content of malondialdehyde (MDA). Furthermore, the antioxidant enzyme activities were obviously enhanced. However, the higher concentration of SNP (100 μmol L−1) enhanced the effects of drought stress for plant. For further analysis of the response mechanism to exogenous NO, the methylation-sensitive amplified polymorphism (MSAP) technique was used to investigate the changes of DNA methylation. When the seedlings of Dendrobium huoshanense were treated with 50 μmol L−1 SNP containing 10% PEG-6000, levels of global DNA methylation of Dendrobium huoshanense were decreased. Nevertheless, the demethylation rate of methylated sites increased, accounting for 12.5% of total methylation sites. These results implied that some expressed genes were involved in the response process to drought stress triggered by NO in Dendrobium huoshanense.
KeywordsDendrobium huoshanense Exogenous nitric oxide Methylation sensitive amplified polymorphism (MSAP) Drought stress DNA methylation Sodium nitroprusside (SNP)
Methylation-sensitive amplified polymorphism
Murashige and Skoog
The present investigation is financially supported by Anhui Provincial Natural Science Foundation for Distinguished Young Scholars (10040606Q22), the University Excellent Young Talents Foundation of Anhui Province (2011SQRL053) and Anhui agricultural University Young Talents Foundation (yj2008-27).
- Fan HH, Li TC, Li ZP, Lin Y, Cai YP (2008) Effects of exogenous nitric oxide on the chlorophyll fluorescence and antioxidant systme of Dendrobium huoshanense under high light stress. Acta Hortic Sini 35(8):1215–1220 (in Chinese with English abstract)Google Scholar
- Kolbert Z, Bartha B, Erdei L (2005) Generation of nitric oxide in roots\of Pisum sativum, Triticum aestivum and Petroselinum crispumplants under osmotic and drought stress. Acta Biol Szegediensis 49:13–16Google Scholar
- Misra AN, Misra M, Singh R (2011) Nitric oxide ameliorates stress responses in plants. Plant Soil Environ 57(3):95–100Google Scholar
- Patade VK, Bhargava S, Suprasanna P (2011) Effects od NaCl and iso-osmotic PEG stress on growth, osmolytes accumulation and antioxidant defense in cultured sugarcane cells. Plant Cell Tiss Organ Cult. doi: 10.1007/s11240-011-0041-5
- Petrivalsky M, Vanickova P, Ryzi M, Navratilova B, Piterkova J, Sedlarova M, Luhova L (2011) The effects of reactive nitrogen and oxygen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts. Plant Cell Tiss Organ Cult. doi: 10.1007/s11240-011-0035-3
- Siddiqui MH, Al-Whaibi MH, Basalah MO (2010) Role of nitric oxide in tolerance of plants to abiotic stress. Protoplasma. doi: 10.1007/s00709-010-0206-9
- Sun J, Li LS, Liu MQ, Wang MJ, Ding MQ, Deng SR, Lu CF, Zhou XY, Shen X, Zheng XJ, Chen SL (2010) Hydrogen peroxide and nitric oxide mediate K+/Na+ homeostasis and antioxidant defense in NaCl-stressed callus cells of two contrasting poplars. Plant Cell Tiss Organ Cult 103:205–215CrossRefGoogle Scholar
- Wang WS, Pan YJ, Zhao XQ, Dwivedi D, Zhu LH, Ali J, Fu BY, Li ZK (2011) Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.). J Exp Bot 62(6):1951–1960Google Scholar