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Somatic embryogenesis in Jarilla heterophylla (Caricaceae)

  • Alejandro Nuño-Ayala
  • Benjamín Rodríguez-Garay
  • Antonia Gutiérrez-MoraEmail author
Original Paper

Abstract

It is reported for the first time an efficient protocol for indirect somatic embryogenesis and plant regeneration for Jarilla heterophylla (Caricaceae). Initial explants were mature zygotic embryos obtained from seeds collected in two regions of the state of Jalisco, México (San Luis Soyatlán and Zacoalco de Torres). Two protocols for somatic embryogenesis used previously for Carica papaya were evaluated. The addition of 2,4-dichlorophenoxyacetic acid (2,4-D) to induction medium A1 without the addition of adenine hemisulphate yielded the best production of embryogenic callus with the higher total of somatic embryos per explant. With this protocol the plant regeneration process took about five months.

Keywords

Carica papaya Adenine hemisulphate Granadina Jarrilla Micropropagation 

Notes

Acknowledgments

Alejandro Nuño-Ayala was a student at the Posgrado en Procesos Biotecnológicos, Centro Universitario de Ciencias Exactas e Ingenierías. Universidad de Guadalajara, México. The authors wish to thank Elizabeth Sugg for english corrections.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Alejandro Nuño-Ayala
    • 1
  • Benjamín Rodríguez-Garay
    • 2
  • Antonia Gutiérrez-Mora
    • 2
    Email author
  1. 1.Unima Soluciones Naturales S.A. de C.V. Santo Tomás de Aquino 5715ZapopanMéxico
  2. 2.Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ, A.C.)GuadalajaraMéxico

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