Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 108, Issue 2, pp 251–256 | Cite as

Doubled haploid plants following colchicine treatment of microspore-derived embryos of oilseed rape (Brassica napus L.)

  • Payam Pour Mohammadi
  • Ahmad MoieniEmail author
  • Asa Ebrahimi
  • Farzad Javidfar
Original Paper


An efficient method for producing doubled haploid plants of oilseed rape (Brassica napus L.) was established using in vitro colchicine treatment of haploid embryos. Haploid embryos in the cotyledonary stage were treated with one of four colchicine concentrations (125, 250, 500 and 1,000 mg/L); for one of three treatment durations (12, 24 and 36 h) at one of the two temperatures (8 and 25°C) and were compared to control embryos (without colchicine treatment). The number of chromosomes, seed recovery, size and density of leaf stomata, and pollen grain size from regenerated plants were determined. No doubled haploid plants were regenerated from control embryos; however, the doubled haploid plants were regenerated from colchicine-treated embryos. A high doubling efficiency, 64.29 and 66.66% of regenerated plants, was obtained from 250 mg/L colchicine treatment for 24 h and 500 mg/L colchicine treatment for 36 h, respectively, at 8°C. Following 500 mg/L colchicine treatment for 36 h, a few plants regenerated (9 plants). At the higher colchicine concentration (1,000 mg/L), no plant regenerated. These results indicate that the colchicine treatment of embryos derived from microspores can induce efficient chromosome doubling for the production of doubled haploid lines of oilseed rape.


Colchicine Chromosome doubling Microspore culture Embryo Oilseed rape Brassicanapus 


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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Payam Pour Mohammadi
    • 1
  • Ahmad Moieni
    • 1
    Email author
  • Asa Ebrahimi
    • 2
  • Farzad Javidfar
    • 3
  1. 1.Department of Plant Breeding, Faculty of AgricultureTarbiat Modares UniversityTehranIran
  2. 2.Faculty of Agriculture and Natural Resources, Science and Research BranchAzad Islamic UniversityTehranIran
  3. 3.Seed and Plant Improvement Research InstituteKarajIran

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