Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 108, Issue 1, pp 121–128 | Cite as

Factors influencing plant regeneration from seedling explants of Hairy nightshade (Solanum sarrachoides)

Original Paper

Abstract

Hairy nightshade (Solanum sarrachoides) has the potential to be a model system for the study of plant-pathogen interactions, however, the availability of tissue culture and transformation methods would strengthen its utility. For the development of tissue culture methods, we investigated, explant type (cotyledons, hypocotyls, roots), hypocotyl explant origin, cotyledon orientation (abaxial vs. adaxial) in direct contact with the medium, gelling agents (agar and agargel) and cytokinins (zeatin and 6-benzyladenine) at different concentrations. Cotyledon explants resulted in the greatest biomass as compared to root and hypocotyl. As for hypocotyl explant origin, explants proximal to the cotyledons had a significant effect on plant regeneration. However, cotyledon orientation and gelling agent had no effect on plant regeneration. Medium supplemented with either zeatin or 6-benzyladenine at 1 mg L−1 resulted in significant shoot regeneration. Shoots rooted readily when cultured on a non-hormone based rooting medium.

Keywords

Model plant Solanaceae Zeatin Benzylamino purine (BAP) Potato leafroll virus (PLRV) 

Supplementary material

11240_2011_20_MOESM1_ESM.ppt (174 kb)
Supplementary Fig. 1 Effect of gelling agent on percent of explants with shoots (A) and the number of shoots per explant (C). Effect of explant origin on percent of explants with shoots (B) and the number of shoots per explant (D). Means from 4 replicates (A and B) and from 3 replicates (C and D). Bars represent standard error (±). (PPT 174 kb)

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Ho-Jong Ju
    • 1
    • 4
    • 5
  • Joyce Van Eck
    • 2
  • Stewart M. Gray
    • 1
    • 3
  1. 1.Department of Plant Pathology and Plant Microbe BiologyCornell UniversityIthacaUSA
  2. 2.The Boyce Thompson Institute for Plant Research, Inc.IthacaUSA
  3. 3.USDA/ARS, Biological Integrated Pest Management Research UnitIthacaUSA
  4. 4.Department of Agricultural BiologyChonbuk National UniversityJeonju-city JeonbukRepublic of Korea
  5. 5.Plant Medicinal Research CenterChonbuk National UniversityJeonju-city JeonbukRepublic of Korea

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