Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 108, Issue 1, pp 73–81 | Cite as

The effects of jasmonic acid and methyl jasmonate on rosmarinic acid production in Mentha × piperita cell suspension cultures

  • Justyna Krzyzanowska
  • Anna Czubacka
  • Lukasz Pecio
  • Marcin Przybys
  • Teresa Doroszewska
  • Anna Stochmal
  • Wieslaw Oleszek
Original Paper

Abstract

The effects of two elicitors: jasmonic acid and methyl jasmonate on cell growth as well as on rosmarinic acid accumulation in cell suspension cultures of Mentha × piperita were investigated. The highest rosmarinic acid accumulation 117.95 mg g−1 DW (12% DW) was measured 24 h after addition of 100 μM methyl jasmonate. A similar concentration 110.12 mg g−1 DW was detected 48 h after application of 200 μM jasmonic acid. Those values were nearly 1.5 times higher compared to the control sample, without elicitation. There was no substantial influence of elicitors on rosmarinic acid secretion into the culture media. Extracellular concentrations of rosmarinic acid were similar to the values from the control variants. It was documented that suspension cultures of M. piperita treated with elicitors showed a decrease in biomass accumulation when compared to the control.

Keywords

Mentha × piperita Cell suspension cultures Jasmonic acid Methyl jasmonate Elicitation Rosmarinic acid 

Abbreviations

2,4-D

2,4-Dichlorophenoxyacetic acid

2iP

6-γ,γ-Dimethylallylaminopurine

JA

Jasmonic acid

MeJA

Methyl jasmonate

RA

Rosmarinic acid

LS

Linsmayer and Skoog medium

HCOOH

Formic acid

UPLC

Ultra performance liquid chromatography

SPE

Solid phase extraction

Notes

Acknowledgments

The work was performed under the 6th Framework Program of European Union NUTRA-SNACKS project.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Justyna Krzyzanowska
    • 1
  • Anna Czubacka
    • 2
  • Lukasz Pecio
    • 1
  • Marcin Przybys
    • 2
  • Teresa Doroszewska
    • 2
  • Anna Stochmal
    • 1
  • Wieslaw Oleszek
    • 1
  1. 1.Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant CultivationState Research InstitutePulawyPoland
  2. 2.Department of Plant Breeding and Biotechnology, Institute of Soil Science and Plant CultivationState Research InstitutePulawyPoland

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