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The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis

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Abstract

The R2R3-Myb proteins comprise a large family of plant transcription factors that regulate the expression of multiple drought-responsive and cold-responsive genes by binding special cis-elements at the promoter. There have been previous studies in transgenic plant over-expression of the myb protein. In this article, we analyzed the function of MdMYB6, an R2R3-type Myb transcription factor from apples (Malus × domestica), in transgenic Arabidopsis plants. In contrast to wild type plants, these transgenic lines accumulated less anthocyanin. Equimolar concentrations of sorbitol did not alter the anthocyanin accumulation. Real-time polymerase chain reaction (PCR) indicated that MdMYB6 over-expression suppressed enzymes involved in anthocyanin biosynthesis and some Arabidopsis basic helix-loop-helix (bHLH) genes. The MdMYB6 transcription faction may be an important repressor of anthocyanin biosynthesis in plants.

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Acknowledgments

This research was supported by the Shanghai National Natural Science Foundation (30670179, 30800602, 08ZR1417200); 863 Program (2006AA10Z117, 2006AA06Z358, 2008AA10Z401); Shanghai Project for ISTC (08540706500); The Key Project Fund of the Shanghai Municipal Committee of Agriculture (No. 2008-7-5); and The Shanghai Rising-Star Program (08QH14021).

Author information

Correspondence to Quan-Hong Yao.

Additional information

J.-J. Gao and X.-F. Shen contributed equally to this article.

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Gao, J., Shen, X., Zhang, Z. et al. The myb transcription factor MdMYB6 suppresses anthocyanin biosynthesis in transgenic Arabidopsis. Plant Cell Tiss Organ Cult 106, 235–242 (2011). https://doi.org/10.1007/s11240-010-9912-4

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Keywords

  • MdMYB6
  • Anthocyanins
  • BHLH
  • Sucrose
  • MYB