Advertisement

Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae)

  • Renata Garcia
  • Georgia Pacheco
  • Erica Falcão
  • Gabriela Borges
  • Elisabeth MansurEmail author
Original Paper

Abstract

Passiflora suberosa is used in popular medicine, improvement programs, and as an ornamental plant. The goal of this study was to establish efficient protocols for plant regeneration and callus induction from nodal, internodal and leaf segments excised from in vitro-grown plants. The different morphogenetic responses were modulated by the type and concentration of plant growth regulators, according to the basal medium and light conditions. Shoot formation occurred through three pathways: (1) development of preexisting meristems, (2) direct organogenesis, and (3) indirect organogenesis. Development of preexisting meristems was observed from nodal segments (1 shoot/explant) in response to α-naphthaleneacetic acid (NAA), picloram (PIC), and 2,4-dichlorophenoxyacetic acid (2,4-D), using two basal media (MS and MSM). Direct organogenesis in this species was obtained for the first time in this work, through shoot development from internodal segments in the presence of 6-benzyladenine (BA). The highest regeneration rates were achieved on MSM medium, regardless of the BA concentration. Indirect organogenesis was achieved from all explant types on media supplemented with BA, used alone or in combination with NAA. The highest regeneration efficiency was obtained from internodal segments cultured on MSM medium plus 44.4 μM BA. Compact, friable, or mucilaginous non-morphogenic calluses were induced by thidiazuron, PIC, 2,4-D, and NAA. High-yielding friable calluses obtained on MSM medium supplemented with 28.9 μM PIC are being used for the establishment of suspension cultures and further analysis of the production of bioactive compounds.

Keywords

Passion fruit Morphogenesis Micropropagation Tissue culture 

Notes

Acknowledgments

The authors acknowledge the Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) for a doctoral scholarship and financial support. E. Mansur is a recipient of a research fellowship from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

References

  1. Becerra DC, Forero AP, Góngora GA (2004) Age and physiological condition of donor plants affect in vitro morphogenesis in leaf explants of Passiflora edulis f. flavicarpa. Plant Cell Tiss Org Cult 79:90–97CrossRefGoogle Scholar
  2. D’Onofrio C, Morini S (2005) Development of adventitious shoots from in vitro grown Cydonia oblonga leaves as influenced by different cytokinins and treatment duration. Biol Plantarum 49:17–21CrossRefGoogle Scholar
  3. Dornelas MC, Vieira MLC (1994) Tissue culture studies on species of Passiflora. Plant Cell Tiss Org Cult 36:211–217CrossRefGoogle Scholar
  4. Drew RA (1991) In vitro culture of adult and juvenile bud explants of Passiflora species. Plant Cell Tiss Org Cult 26:23–27CrossRefGoogle Scholar
  5. Faria JLC, Segura J (1997) In vitro control of adventitious bud differentiation by inorganic medium components and silver thiosulfate in explants of Passiflora edulis f. flavicarpa. In Vitro Cell Dev Biol Plant 33:209–212CrossRefGoogle Scholar
  6. Ferreira G, Oliveira A, Rodrigues JD, Dias GB, Detoni AM, Tesser SM, Antunes AM (2005) Effect of aril in Passiflora alata seed germination in different substrates and submitted to previous germination treatments with gibberellin. Rev Bras Frut 27:277–280CrossRefGoogle Scholar
  7. Freitas IMM (1997) Micropropagation of passionfruit. Act Hortic 28:103–106Google Scholar
  8. Gardner DE (1989) Pathogenecity of Fusarium oxyporum f. sp. passiflorae to Banana Poka and other Passiflora spp. in Hawaii. Plant Diasease 73:476–478CrossRefGoogle Scholar
  9. Junqueira NTV, Braga MF, Faleiro FG, Peixoto JR, Bernacci LC (2005) Potential of wild species of passionfruit as sources of resistance to diseases. In: Faleiro FG, Junqueira NTV, Braga MF (eds) Passionfruit: germplasm and breeding. Embrapa Cerrados, Planaltina, pp 143–148Google Scholar
  10. Kantharajah AS, Dodd WA (1990) In vitro micropropagation of Passiflora edulis (purple passionfruit). Ann Bot 48:673–680Google Scholar
  11. Khurana-Kaul V, Kachhwaha S, Kothari SL (2010) Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium. Biol Plantarum 54:369–372CrossRefGoogle Scholar
  12. Kidoy L, Nygaard AM, Andersen OM, Pedersen AT, Aksnes DW, Kiremire BT (1997) Anthocyanins in fruits of Passiflora edulis and P. suberosa. J Food Compos Anal 10:49–54CrossRefGoogle Scholar
  13. Lombardi SP, Passos IRS, Nogueira MCS, Appezzato-da-Glória B (2007) In vitro shoot regeneration from roots and leaf discs of Pasiflora cincinnata Mast. Braz Arch Biol Technol 50:239–247CrossRefGoogle Scholar
  14. Miller LG (1998) Herbal medicinals: selected clinical considerations focusing on known or potential drug-herb interactions. Arch Intern Med 158(20):2200–2211PubMedCrossRefGoogle Scholar
  15. Monteiro ACBA, Higashi EN, Gonçalves AN, Rodriguez APM (2000a) A novel approach for the definition of the inorganic medium components for micropropagation of yellow passionfruit (Passiflora edulis Sims. f. flavicarpa Deg.). In Vitro Cell Dev Biol Plant 36:527–531Google Scholar
  16. Monteiro ACBA, Nakazawa GT, Mendes BMJ, Rodriguez APM (2000b) In vitro regeneration of Passiflora suberosa from leaf discs. Sci Agric 57:571–573CrossRefGoogle Scholar
  17. Moran Robles MJ (1978) In vitro vegetative multiplication of axillary buds of P. edulis var. flavicarpa Degener and P. mollissima Bairley. Fruits 33:701–715Google Scholar
  18. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  19. Osipi EAF, Nakagawa J (2005) Effects of temperature on evaluation of physiological quality of seeds of sweet passionfruit (Passiflora alata Dryander). Rev Bras Frutic 27:179–181CrossRefGoogle Scholar
  20. Otoni WC, Casali VWD, Power JB, Davey MR (1996) Protoplast isolation from mesophyll of P. suberosa L.: influence of age of donor plant. Rev Ceres 43:157–164Google Scholar
  21. Passos IRS, Bernacci LC (2005) Tissue culture applied to in vitro germoplasm maintenance and genetic improvement of passion fruit (Passiflora spp.). In: Faleiro FG, Junqueira NTV, Braga MF (eds) Passionfruit: germplasm and breeding. Embrapa, Brasília, pp 361–383Google Scholar
  22. Pinto APC, Monteiro-Hara ACBA, Stipp LCL, Mendes BMJ (2010) In vitro organogenesis of Passiflora alata. In Vitro Cell Dev Biol Plant 46:28–33Google Scholar
  23. Spencer KC, Segler DS (1987) Passisuberosin and epipassisuberosin: two cyclopentenoid cyanogenic glycosides from P. suberosa. Phytochemistry 26:1665–1667CrossRefGoogle Scholar
  24. Trevisan F, Mendes BMJ (2005) Optimization of in vitro organogenesis in passion fruit (Passiflora edulis f. flavicarpa). Sci Agric 62:346–350CrossRefGoogle Scholar
  25. Ulmer T, MacDougal JM (2004) Passiflora passionflowers of the world. Timber Press, PortlandGoogle Scholar
  26. Vanderplank J (1996) Passion flowers. MIT Press, MassachusettsGoogle Scholar
  27. Vieira MLC, Carneiro MS (2004) Passiflora spp., passionfruit. In: Litz RE (ed) Biotechnology of fruit and nut crops. CABI Publishing, Oxford, pp 435–453Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Renata Garcia
    • 1
    • 2
  • Georgia Pacheco
    • 2
  • Erica Falcão
    • 2
  • Gabriela Borges
    • 1
    • 2
  • Elisabeth Mansur
    • 2
    Email author
  1. 1.Universidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Instituto de Biologia Roberto Alcantara Gomes, Núcleo de Biotecnologia VegetalUniversidade do Estado do Rio de JaneiroRio de JaneiroBrazil

Personalised recommendations