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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 105, Issue 3, pp 383–393 | Cite as

Changes in DNA-methylation during zygotic embryogenesis in interspecific hybrids of beans (Phaseolus ssp.)

  • Ghassen AbidEmail author
  • Yordan Muhoviski
  • Jean-Marie Jacquemin
  • Dominique Mingeot
  • Khaled Sassi
  • André Toussaint
  • Jean-Pierre Baudoin
Original Paper

Abstract

Hybrid embryos resulting from crosses between Phaseolus species often fail to reach maturity and some combinations frequently abort at early developmental stages. The genetic or molecular basis for these consistent developmental defects is at present not clear. However, an extremely complex genetic system, thought to be caused by major epigenetic changes associated with gene expression changes, has been shown to be active in plant species. We have investigated DNA methylation in two interspecific hybrids, Phaseolus vulgaris × Phaseolus coccineus and its reciprocal crosses, using methylation sensitive amplification polymorphism (MSAP). The potential use of MSAP for detecting methylation variation during embryogenesis in interspecific hybrids is discussed. Significant differences in the DNA methylation patterns were observed in abortive (interspecific hybrids) and non abortive (parental) genotypes. Taken together, our results strongly suggest that generalized alterations in DNA methylation profiles could play a causative role in early interspecific embryo abortion in vivo. A considerable change in the methylation pattern during embryogenesis could be involved in the disruption of the regulation or maintenance of the embryogenesis process of Phaseolus interspecific hybrids. The results also support the earlier hypothesis that DNA methylation is critical for the regulation of plant embryogenesis and gene expression.

Keywords

DNA-methylation MSAP Interspecific hybrids Phaseolus vulgaris L. Phaseolus coccineus L. 

Abbreviations

DAP

Days after pollination

MSAP

Methylation sensitive amplification polymorphism

RT–PCR

Reverse transcription polymerase chain reaction

SSR

Simple sequence repeats

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Ghassen Abid
    • 1
    • 2
    Email author
  • Yordan Muhoviski
    • 2
  • Jean-Marie Jacquemin
    • 2
  • Dominique Mingeot
    • 2
  • Khaled Sassi
    • 3
  • André Toussaint
    • 1
  • Jean-Pierre Baudoin
    • 1
  1. 1.University of Liège-Gembloux Agro-Bio Tech Unit of Tropical Crop Husbandry and HorticultureGembloux Agricultural UniversityGemblouxBelgium
  2. 2.Department of Life Sciences, Unit of Breeding and BiodiversityWalloon Agricultural Research CentreGemblouxBelgium
  3. 3.Department of Agronomy and Plant BiotechnologyLaboratory of AgronomyTunisTunisia

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