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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 105, Issue 3, pp 329–335 | Cite as

Effects of light quality on growth and development of protocorm-like bodies of Dendrobium officinale in vitro

  • Yuan Lin
  • Jia Li
  • Bo Li
  • Tao He
  • Ze ChunEmail author
Original Paper

Abstract

The effect of light quality on protocorm-like bodies (PLBs) of Dendrobium officinale was investigated. PLBs of D. officinale were incubated under a number of different light conditions in vitro, namely: dark conditions; fluorescent white light (Fw); red light-emitting diodes (LEDs); blue LEDs; half red plus half blue [RB (1:1)] LEDs; 67% red plus 33% blue [RB (2:1)] LEDs; and 33% red plus 67% blue [RB (1:2)] LEDs. Growth parameters, number of shoots produced per PLB, chlorophyll concentration and carotenoid concentration were measured after 90 days culture. The percentage of PLBs producing shoots was 85% under blue LEDs. In contrast, the percentage of PLBs producing shoots was less than 60% under dark conditions, fluorescent white light and red LEDs. The number of shoots produced per PLB was more than 1.5 times greater under blue LEDs, RB (1:1) LEDs and RB (1:2) LEDs than those cultured under other light treatments [dark, Fw, red LEDs and RB (2:1)]. Chlorophyll and carotenoid concentrations were significantly higher under blue LEDs and different red plus blue LED ratios, compared to other light treatments (dark, Fw and red LEDs). Blue LEDs, Fw, and RB (1:2) LEDs produced higher dry matter accumulations of PLBs and shoots. This study suggests that blue LEDs or RB (1:2) LEDs could significantly promote the production of shoots by protocorm-like bodies of D. officinale and increase the dry matter of PLBs and the accumulation of shoot dry matter in vitro.

Keywords

Dendrobium officinale Protocorm-like bodies (PLBs) Light-emitting diode (LED) Shoot Chlorophyll 

Abbreviations

LED

Light-emitting diode

PLBs

Protocorm-like bodies

Fw

Fluorescent white light

RB(1:1)

Half red LEDs plus half blue LEDs

RB(2:1)

67% red plus 33% blue LEDs

RB(1:2)

33% red plus 67% blue LEDs

PPF

Photosynthesis photon flux

Notes

Acknowledgments

The authors thank Prof. Yu (Center for Agricultural Biotechnology, Chengdu Institute of Biology, Chinese Academy of Sciences) for the use of his facilities. This research was supported by the Science & Technology-based Platform Construction Project of Sichuan Province, National Spark Program of China (2008GA810003), the Science & Technology Pillar Program of Sichuan Province (2009FZ0077) and Chengdu Institute of Biology, Chinese Academy of Sciences Knowledge Innovation Project at the cutting edge.

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Chengdu Institute of BiologyChinese Academy of SciencesChengduChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina

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