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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 105, Issue 1, pp 105–111 | Cite as

Embryo rescue and plant regeneration in banana (Musa spp.)

  • S. Uma
  • S. Lakshmi
  • M. S. Saraswathi
  • A. Akbar
  • M. M. Mustaffa
Original Paper

Abstract

An efficient regeneration protocol for zygotic embryos at varying maturity stages was developed for wild banana (Pisang Jajee (AA)). Embryo ontogeny was studied to determine the best maturity stage for embryo rescue, suitable media and culture conditions (light and dark) for germination and regeneration. The conversion of endosperm from transparent fluid into a semi-solid state was followed by visible embryo development, which commenced only after 70% embryo maturity. Zygotic embryos of Pisang Jajee at different maturity levels were excised and cultured on medium fortified with different concentrations of 6-benzyl adenine (BA) and indole acetic acid (IAA). Zygotic embryos produced callus or plantlets 25 days after initiation. The frequency of callus induction was greater in immature embryos irrespective of the media composition and decreased with increasing maturity. Fully matured embryos regenerated directly into plantlets without producing callus. Immature embryos required medium supplemented with plant growth regulators (PGRs) for successful regeneration. Although the culture conditions had no influence, dark conditions favoured callus induction and plant regeneration.

Keywords

Banana Embryo maturity In vitro culture Zygotic embryo Callus Regeneration 

Abbreviations

BA

6-benzyl adenine

IAA

Indole acetic acid

PGRs

Plant growth regulators

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • S. Uma
    • 1
  • S. Lakshmi
    • 1
  • M. S. Saraswathi
    • 1
  • A. Akbar
    • 1
  • M. M. Mustaffa
    • 1
  1. 1.National Research Center for Banana (NRCB)TrichyIndia

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