Advertisement

Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant

  • Iyyakkannu Sivanesan
  • Ju Yeon Song
  • Seung Jae Hwang
  • Byoung Ryong Jeong
Original Paper

Abstract

A simple and efficient micropropagation system was developed for Cotoneaster wilsonii through node and shoot tip explants obtained from mature field-grown plants. Of the two explants, node explants were found to be the most effective for axillary shoot proliferation. The highest frequency of shoot induction was achieved when nodal explants were incubated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L−1 thidiazuron (TDZ) and 0.1 mg L−1 α- naphthaleneacetic acid (NAA) with an average of 34 shoots per explant. The microshoots were separated from the multiple shoots and subcultured on MS medium supplemented with 3% (w/v) sucrose and 0.8% (w/v) agar for further shoot growth. Maximum rooting was obtained on half-strength MS medium supplemented with 0.5 mg L−1 indole-3-butyric acid (IBA). The in vitro-grown plantlets were successfully acclimatized in a glasshouse with 98% of survival. High concentrations of TDZ (1.5–2.0 mg L−1) and repeated subcultures resulted hyperhydric shoots. Supplementation of the culture medium with silicon significantly reduced the induction of hyperhydric shoots. Increasing silicon concentration significantly decreased malondialdehyde content of the regenerated shoots. Data indicate that addition of silicon to the culture medium can effectively control hyperhydricity.

Keywords

Endangered species Hyperhydricity In vitro propagation Malondialdehyde Silicon Thidiazuron 

Abbreviations

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

EDXA

Energy Dispersive X-ray Analysis

MDA

Malondialdehyde

MS

Murashige and Skoog medium

NAA

α- Naphthaleneacetic acid

SEM

Scanning Electron Microscopy

TBA

Thiobarbituric acid

TDZ

Thidiazuron

Notes

Acknowledgments

This research was supported by Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. Project No: 109096-5. Iyyakkannu Sivanesan and Ju Yeon Song were supported by a scholarship from the BK21 Program, the Ministry of Education, Science and Technology, Korea.

References

  1. Ahmad N, Anis M (2007) Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agrofor Syst 71:195–200CrossRefGoogle Scholar
  2. Apóstolo NM, Llorente BE (2000) Anatomy of normal and hyperhydric leaves and shoots of in vitro grown Simmondsia chinesis (Link) Schn. In Vitro Cell Dev Biol Plant 36:243–249CrossRefGoogle Scholar
  3. Babaoglu M, Yorgancilar M (2000) TDZ-specific plant regeneration in salad burnet. Plant Cell Tissue Organ Cult 63:31–34CrossRefGoogle Scholar
  4. Bartish IV, Hylmö B, Nybom H (2001) RAPD analysis of interspecific relationships in presumably apomictic Cotoneaster species. Euphytica 120:273–280CrossRefGoogle Scholar
  5. Beruto M, Lanteri L, Portogallo C (2004) Micropropagation of tree peony (Paeonia suffruticosa). Plant Cell Tissue Organ Cult 79:249–255CrossRefGoogle Scholar
  6. Bosela MJ (2009) Effects of b-lactam antibiotics, auxins, and cytokinins on shoot regeneration from callus cultures of two hybrid aspens, Populus tremuloides × P. tremula and P. x canescens × P. gradidentata. Plant Cell Tissue Organ Cult 98:249–261CrossRefGoogle Scholar
  7. Bosela MJ, Michler CH (2008) Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro : evaluation of multiple nutrient formulations and cytokinin types. In Vitro Cell Dev Biol Plant 44:316–329CrossRefGoogle Scholar
  8. Caboni E, Tonelli MG, Lauri P, D’Angeli S, Damiano C (1999) In vitro shoot regeneration from leaves of wild pear. Plant Cell Tissue Organ Cult 59:1–7CrossRefGoogle Scholar
  9. Casanova EVA, Valdes AE, Fernandez B, Moysset L, Trillas MI (2004) Levels and immunolocalization of endogenous cytokinins in thidiazuron-induced shoot organogenesis in carnation. J Plant Physiol 161:95–104PubMedCrossRefGoogle Scholar
  10. Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tissue Organ Cult 64:145–157CrossRefGoogle Scholar
  11. Chang CS, Jeon JI (2003) Leaf flavonoids in Cotoneaster wilsonii (Rosaceae) from the island Ulleung-do, Korea. Biochem Syst Ecol 31:171–179CrossRefGoogle Scholar
  12. Cheruvathur KM, Abraham J, Mani B, Thomas TD (2010) Adventitious shoot induction from cultured internodal explants of Malaxis acuminata D. Don, a valuable terrestrial medicinal orchid. Plant Cell Tissue Organ Cult 101:163–170CrossRefGoogle Scholar
  13. Cogbill S, Faulcon T, Jones G, McDaniel M, Harmon G, Blackmon R, Young M (2010) Adventitious shoot regeneration from cotyledonary explants of rapid-cycling fast plants of Brassica rapa L. Plant Cell Tissue Organ Cult 101:127–133CrossRefGoogle Scholar
  14. Dewir YH, Chakrabarty D, Ali MB, Hahn EJ, Paek KY (2006) Lipid peroxidation and antioxidant enzyme activities of Euphorbia millii hyperhydric shoots. Environ Exp Bot 58:93–99CrossRefGoogle Scholar
  15. Epstein E (1999) Silicon. Annu Rev Plant Physiol Plant Mol Biol 50:641–664PubMedCrossRefGoogle Scholar
  16. Eraslan F, Inal A, Pilbeam DJ, Gunes A (2008) Interactive effects of salicylic acid and silicon on oxidative damage and antioxidant activity in spinach (Spinacia oleracea L. cv. Matador) grown under boron toxicity and salinity. Plant Growth Regul 55:207–219CrossRefGoogle Scholar
  17. Feng JC, Yu XM, Shang XL, Li JD, Wu YX (2010) Factors influencing efficiency of shoot regeneration in Ziziphus jujuba Mill. ‘Huizao’. Plant Cell Tissue Organ Cult 101:111–117CrossRefGoogle Scholar
  18. Francis SV, Senapati SK, Rout GR (2007) Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plant. In Vitro Cell Dev Biol Plant 43:140–143CrossRefGoogle Scholar
  19. Goncalves S, Fernandes L, Romano A (2010) High-frequency in vitro propagation of the endangered species Tuberaria major. Plant Cell Tissue Organ Cult 101:359–363CrossRefGoogle Scholar
  20. Gong HJ, Randall DP, Flowers TJ (2006) Silicon deposition in the root reduces sodium uptake in rice (Oryza sativa L.) seedlings by reducing bypass flow. Plant Cell Environ 29:1970–1979PubMedCrossRefGoogle Scholar
  21. Gray DJ, Benton CM (1991) In vitro micropropagation and plant establishment of muscadine grape cultivars (Vitis rotundifolia). Plant Cell Tissue Organ Cult 27:7–14CrossRefGoogle Scholar
  22. Gunes A, Inal A, Alpaslan M, Eraslan F, Bagci EG, Cicek N (2007a) Salicylic acid induced changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. J Plant Physiol 164:728–736PubMedCrossRefGoogle Scholar
  23. Gunes A, Inal A, Bagci EG, Coban S, Sahin O (2007b) Silicon increases boron tolerance and reduces oxidative damage of wheat grown in soil with excess boron. Biol Plant 51:571–574CrossRefGoogle Scholar
  24. Gunes A, Inal A, Bagci EG, Coban S, Pilbeam DJ (2007c) Silicon mediates changes to some physiological and enzymatic parameters symptomatic for oxidative stress in spinach (Spinacia oleracea L.) grown under B toxicity. Sci Hortic 113:113–119CrossRefGoogle Scholar
  25. Gupta SD, Prasad VSS (2010) Shoot multiplication kinetics and hyperhydric status of regenerated shoots of gladiolus in agar-solidified and matrix-supported liquid cultures. Plant Biotechnol Rep 4:85–94CrossRefGoogle Scholar
  26. He SS, Liu CZ, Saxena PK (2007) Plant regeneration of an endangered medicinal plant Hydrastis canadensis L. Sci Hortic 113:82–86CrossRefGoogle Scholar
  27. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119CrossRefGoogle Scholar
  28. Jaiswal S, Sawhney S (2006) Modulation of TDZ-induced morphogenetic responses by anti-auxin TIBA in bud-bearing foliar explants of Kalanchoe pinnata. Plant Cell Tissue Organ Cult 86:69–76CrossRefGoogle Scholar
  29. Joshi P, Dhawan V (2007) Axillary multiplication of Swertia chirayita (Roxb. Ex Fleming) H. Karst., a critically endangered medicinal herb of temperate Himalayas. In Vitro Cell Dev Biol Plant 43:631–638CrossRefGoogle Scholar
  30. Kadota M, Niimi Y (2003) Effects of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tissue Organ Cult 72:261–265CrossRefGoogle Scholar
  31. Kevers C, Franck T, Strasser RJ, Dommes J, Gaspar T (2004) Hyperhydricity of micropropagated shoots: a typically stress-induced change of physiological state. Plant Cell Tissue Organ Cult 77:181–191CrossRefGoogle Scholar
  32. Kim YS (2006) Conservation of plant diversity in Korea. Landscape Ecol Eng 2:163–170CrossRefGoogle Scholar
  33. Kim MS, Schumann CM, Klopfenstein NB (1997) Effects of thidiazuron and benzyladenine on axillary shoot proliferation of three green ash (Fraxinus pennsylvanica Marsh.) clones. Plant Cell Tissue Organ Cult 48:45–52CrossRefGoogle Scholar
  34. Li X, Ahlman A, Yan X, Lindgren H, Zhu LH (2010) Genetic transformation of the oilseed crop Crambe abyssinica. Plant Cell Tissue Organ Cult 100:149–156CrossRefGoogle Scholar
  35. Liang Y (1999) Effects of silicon on enzyme activity and sodium, potassium and calcium concentration in barley under salt stress. Plant Soil 209:217–224CrossRefGoogle Scholar
  36. Liang Y, Zhang W, Chen Q, Liu Y, Ding R (2006) Effect of exogenous silicon (Si) on H + -ATPase activity. phospholipids and fluidity of plasma membrane in leaves of salt-stressed barley (Hordeum vulgare L.). Environ Exp Bot 57:212–219CrossRefGoogle Scholar
  37. Liang Y, Sun W, Zhu YG, Christie P (2007) Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ Pollut 147:422–428PubMedCrossRefGoogle Scholar
  38. Ma JF, Yamaji N (2006) Silicon uptake and accumulation in higher plants. Trends Plant Sci 11:392–397PubMedCrossRefGoogle Scholar
  39. Monier C, Ochatt SJ (1995) Establishing micropropagation conditions for five Cotoneaster genotypes. Plant Cell Tissue Organ Cult 42:275–281CrossRefGoogle Scholar
  40. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  41. Norton ME, Boe AA (1982) In vitro propagation of ornamental rosaceous plants. Hortic Sci 17:190–191Google Scholar
  42. Noshad D, Miresmaili S, Riseman A, Ekramoddoullah A (2009) In vitro propagation of seven Daphne L. species. Plant Cell Tissue Organ Cult 96:201–209CrossRefGoogle Scholar
  43. Ochatt SJ, Muneaux E, Machado C, Jacas L, Pontecaille C (2002) The hyperhydricity of in vitro regenerants of grass pea (Lathyrus sativus L.) is linked with an abnormal DNA content. J Plant Physiol 159:1021–1028CrossRefGoogle Scholar
  44. Offord CA, Tyler JL (2009) In vitro propagation of Pimelea spicata R.Br (Thymelaeaceae), an endangered species of the Sydney region, Australia. Plant Cell Tissue Organ Cult 98:19–23CrossRefGoogle Scholar
  45. Olmos E, Hellín E (1998) Ultrastructural differences of hyperhydric and normal leaves from regenerated carnation plants. Sci Hortic 75:91–101CrossRefGoogle Scholar
  46. Pérez-Tornero O, Egea J, Olmos E, Burgos L (2001) Control of hyperhydricity in micropropagated apricot cultivars. In Vitro Cell Dev Biol Plant 37:250–254CrossRefGoogle Scholar
  47. Piqueras A, Han BH, Van Huylenbroeck JM, Debergh PC (1998) Effect of different environmental conditions in vitro on sucrose metabolism and antioxidant enzymatic activities in cultured shoots of Nicotiana tabacum L. Plant Growth Regul 25:5–10CrossRefGoogle Scholar
  48. Piqueras A, Cortina M, Serna MD, Casas JL (2002) Polyamines and hyperhydricity in micropropagated carnation plants. Plant Sci 162:671–678CrossRefGoogle Scholar
  49. Ranganathan S, Suvarchala V, Rajesh YBRD, Prasad SM, Padmakumari AP, Voleti SR (2006) Effects of silicon sources on its deposition, chlorophyll content, and disease and pest resistance in rice. Biol Plant 50:713–716CrossRefGoogle Scholar
  50. Ray A, Bhattacharya S (2008) An improved micropropagation of Eclipta alba by in vitro priming with chlorocholine chloride. Plant Cell Tissue Organ Cult 92:315–319CrossRefGoogle Scholar
  51. Rodrigues FÁ, Vale FXR, Korndörfer GH, Prabhu AS, Datnoff LE, Oliveira AMA, Zambolim L (2003) Influence of silicon on sheath blight of rice in Brazil. Crop Prot 22:23–29CrossRefGoogle Scholar
  52. Saher S, Piqueras A, Hellin E, Olmos E (2004) Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress. Physiol Plant 120:152–161PubMedCrossRefGoogle Scholar
  53. Shi G, Cai Q, Liu C, Wu L (2010) Silicon alleviates cadmium toxicity in peanut plants in relation to cadmium distribution and stimulation of antioxidative enzymes. Plant Growth Regul 61:45–52CrossRefGoogle Scholar
  54. Sivanesan I, Jeong BR (2007) Direct shoot regeneration from nodal explants of Sida cordifolia Linn. In Vitro Cell Dev Biol Plant 43:436–441CrossRefGoogle Scholar
  55. Steinitz B, Küsek M, Tabib Y, Paran I, Zelcer A (2003) Pepper (capsicum annuum L.) regenerants obtained by direct somatic embryogenesis fail to develop a shoot. In Vitro Cell Dev Biol Plant 39:296–303CrossRefGoogle Scholar
  56. Thimmappaiah, Shirly RA, Sadhana PH (2002). In vitro propagation of cashew from young trees. In Vitro Cell Dev Biol Plant 38:152–156Google Scholar
  57. Wang YL, Wang XD, Zhao B, Wang YC (2007) Reduction of hyperhydricity in the culture of Lepidium meyenii shoots by the addition of rare earth elements. Plant Growth Regul 52:151–159CrossRefGoogle Scholar
  58. Whitehouse AB, Marks TR, Edwards GA (2002) Control of hyperhydricity in Eucalyptus axillary shoot cultures grown in liquid medium. Plant Cell Tissue Organ Cult 71:245–252CrossRefGoogle Scholar
  59. Wu Z, Chen LJ, Long YJ (2009) Analysis of ultrastructure and reactive oxygen species of hyperhydric garlic (Allium sativum L.) shoots. In Vitro Cell Dev Biol Plant 45:483–490Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Iyyakkannu Sivanesan
    • 1
  • Ju Yeon Song
    • 1
  • Seung Jae Hwang
    • 2
  • Byoung Ryong Jeong
    • 1
    • 3
  1. 1.Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate SchoolGyeongsang National UniversityJinjuKorea
  2. 2.Department of HorticultureGyeongsang National UniversityJinjuKorea
  3. 3.Institute of Agriculture and Life ScienceGyeongsang National UniversityJinjuKorea

Personalised recommendations