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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 104, Issue 2, pp 157–162 | Cite as

Shoot organogenesis and somatic embryogenesis from leaf and shoot explants of Ochna integerrima (Lour)

  • Guohua MaEmail author
  • Jinfeng Lü
  • Jaime A. Teixeira da Silva
  • Xinhua Zhang
  • Jietang Zhao
Original paper

Abstract

Ochna integerrima is a medicinal and ornamental plant in Southeastern Asia. It has been listed as a rare and endangered species in China. Here we studied the effects of plant growth regulators and their concentrations on the induction of somatic embryogenesis and shoot organogenesis from leaf and shoot explants of O. integerrima for the first time. Cytokinins played a crucial role in somatic embryogenesis and shoot organogenesis. Among them, a higher concentration of thidiazuron (10.0–15.0 μM TDZ) could induce both somatic embryogenesis and adventitious shoot formation whereas low concentrations of TDZ (5.0 μM) could only induce adventitious shoots. However, 6-benzyladenine (BA at 5–15 μM) could only induce adventitious shoots. Shoot explants induced more adventitious shoots and somatic embryos than leaf explants when cultured on medium with the same concentration (5–15 μM) of TDZ or 15 μM BA. Medium containing 0.5 μM α-naphthaleneacetic acid and 8 μM indole-3-butyric acid and 0.1% activated charcoal could induce adventitious roots within 1 month. An efficient mass propagation and regeneration system has been established.

Keywords

Ochna integerrima TDZ Shoot organogenesis Somatic embryogenesis 

Abbreviations

2,4-D

2,4-dichloro-phenoxyacetic acid

BA

6-benzyladenine

IBA

Indole-3-butyric acid

NAA

α-naphthaleneacetic acid

TDZ

Thidiazuron

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Guohua Ma
    • 1
    Email author
  • Jinfeng Lü
    • 1
    • 2
  • Jaime A. Teixeira da Silva
    • 3
  • Xinhua Zhang
    • 1
  • Jietang Zhao
    • 1
  1. 1.Key Laboratory of Plant Resources Conservation and Sustainable UtilizationSouth China Botanical Garden, The Chinese Academy of SciencesGuangzhouChina
  2. 2.Graduate University of Chinese Academy of SciencesBeijingChina
  3. 3.Faculty of Agriculture and Graduate School of AgricultureKagawa UniversityKagawa-kenJapan

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