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In vitro regeneration in Dierama erectum Hilliard

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Abstract

The genus Dierama comprises plants with a potential to be developed as ornamentals. D. erectum seeds were decontaminated and germinated on 1/10th strength Murashige and Skoog (Physiol Plant 15:473–497, 1962) (MS) media without plant growth regulators or sucrose. In an experiment investigating the effects of 6-benzyladenine (BA), meta-Topolin (mT), kinetin (KIN) and zeatin (Z) with or without α-naphthaleneacetic acid (NAA), the highest shoot number per hypocotyl (4.20 ± 0.51) was obtained from MS medium supplemented with 1.0 μM Z after 8 weeks. This was followed by a combination of 2.0 μM KIN and 2.0 μM NAA with 3.67 ± 0.81 shoots per explant. BA treatments produced 3.20 ± 0.22 shoots per hypocotyl explant when 2.0 μM was combined with 1.0 μM NAA, while mT gave 3.09 ± 0.99 shoots per explant when 2.0 μM mT was combined with 2.0 μM NAA. Adventitious shoot regeneration was optimised when shoots were grown under a 16-h photoperiod at 100 μmol m−2 s−1 on MS medium supplemented with 1.0 μM BA. This resulted in an average of 12.73 ± 1.03 shoots per hypocotyl explant. Various concentrations of ancymidol, activated charcoal and sucrose did not promote in vitro corm formation of this species. Plants rooted successfully after 8 weeks on MS medium supplemented with 1.0 μM indole-3-butyric acid (IBA) and had an average root number of 2.73 ± 0.40. After 2 months of acclimatisation, plants had formed corms. The largest corms (of diameter 0.45 ± 0.03 cm) were produced in plants pre-treated with 0.5 μM IBA. The highest plant survival percentage of 73% was also associated with this treatment.

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Fig. 4a–e

Abbreviations

ANOVA:

Analysis of variance

BA:

6-benzyladenine

DMRT:

Duncan’s multiple range test

HCl:

Hydrochloric acid

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

KIN:

Kinetin

MS:

Murashige and Skoog medium

mT:

meta-Topolin

NAA:

α-naphthaleneacetic acid

PGR:

Plant growth regulator

KOH:

Potassium hydroxide

NaOCl:

Sodium hypochlorite

PPFD:

Photosynthetic photon flux density

Z:

Zeatin

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Acknowledgements

We thank the National Research Foundation, Pretoria, and the National Manpower Development Secretariat (NMDS) of the Lesotho Government for their generous financial assistance. Our sincere gratitude also goes to Dr. Gary Stafford for helping with the seed collection.

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Correspondence to J. Van Staden.

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Koetle, M.J., Finnie, J.F. & Van Staden, J. In vitro regeneration in Dierama erectum Hilliard. Plant Cell Tiss Organ Cult 103, 23–31 (2010). https://doi.org/10.1007/s11240-010-9749-x

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Keywords

  • Cytokinins
  • Dierama erectum
  • Micropropagation
  • Photoperiod
  • Threatened species