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Regeneration of somatic hybrids of ginger via chemical protoplast fusion

Abstract

Ginger (Zingiber officinale Rosc.) somatic hybridization was attempted by using polyethylene glycol (PEG)-mediated protoplast fusion. Protoplasts of three ginger cultivars isolated from the embryogenic cell suspensions were fused with each other. The highest binary fusion rate [13.5% in the fusion of ginger ‘Lushan Zhangliang jiang’ + ‘Chenggu Huang Jiang’ (LZ + CH)] was observed with the treatment of 30% PEG6000 for 15 min. The three fusion combinations can efficiently develop into micro-colonies and redifferentiate, but only the fusion of ginger ‘Chenggu Huang Jiang’ + ‘Sichuan Zhugen Jiang’ (CH + SZ) could regenerate plantlets. Approximately 15 months were used for the regeneration of whole plants, and 15 shoots were obtained from the fusion of LZ + CH. Three plantlets were identified as hybrids by using RAPD, and they were all diploids by analysis with flow cytometry.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (30771465) and the National Key Technologies Research and Development (R&D) Program of China (2006BAD13B06).

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Correspondence to Zhenxian Zhang.

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Guan, Q., Guo, Y., Wei, Y. et al. Regeneration of somatic hybrids of ginger via chemical protoplast fusion. Plant Cell Tiss Organ Cult 102, 279–284 (2010). https://doi.org/10.1007/s11240-010-9730-8

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Keywords

  • Ginger
  • Protoplast fusion
  • Somatic hybrid
  • Regeneration