Transient gene expression in rose petals via Agrobacterium infiltration
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The study of gene function in roses is hampered by the low efficiency of transformation systems and the long time span needed for the generation of transgenic plants. For some functional analyses, the transient expression of genes would be an efficient alternative. Based on current protocols for the transient expression of genes via the infiltration of Agrobacterium into plant tissues, we developed a transient expression system for rose petals. We used β-glucuronidase (GUS) as a marker gene to optimize several parameters with effects on GUS expression. The efficiency of expression was found to be dependent on the rose genotype, flower age, position of petals within a flower, Agrobacterium strain and temperature of co-cultivation. The highest GUS expression was recorded in petals of the middle whirls of half-bloomed flowers from cultivars of ‘Pariser Charme’ and ‘Marvel’.
KeywordsRosa Rose petals Agrobacterium mediated transient expression
Transfer deoxyribonucleic acid
The first author is thankful to Deutscher Akademischer Austausch Dienst (DAAD), Higher Education Commission of Pakistan (HEC) and Sindh Agriculture University, Tandojam for the award of scholarship and study leave.
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