Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 102, Issue 2, pp 259–263 | Cite as

Nuclear DNA content of Hydrastis canadensis L. and genome size stability of in vitro regenerated plantlets

  • Samuel G. Obae
  • Todd P. WestEmail author
Research Note


Knowing the genome size is an important step towards deciding and planning for genome sequencing of a given species. Using flow cytometry, nuclear DNA content of Hydrastis canadensis was estimated, and genome size stability of its in vitro regenerated plantlets were assessed. The nuclear DNA content of H. canadensis was estimated to be 2.62 ± 0.020 pg/2C. This is the first report to estimate nuclear DNA content of H. canadensis; therefore this study provides valuable information that will facilitate genome sequencing and subsequent molecular studies of this economically important medicinal plant. Comparison of genome size between in vitro regenerated (explant source was from wild plants) and wild plants of H. canadensis did not reveal any significant difference (P ≤ 0.05) in nuclear DNA content. This suggests that micropropagation of H. canadensis, even after numerous sub-culturing and long-term culture periods produced in vitro plantlets with a stable genome size. These results provide further evidence that micropropagation techniques have the potential to be used as a source of planting stock, along with seeds, for restoring locally threatened H. canadensis wild populations and for commercial cultivation to supply the growing herbal market.


Germplasm conservation Medicinal plant Goldenseal Yellow root Flow cytometry Tissue culture 



Arumuganathan and Earle’s buffer




Flow cytometry


Milli watt


Propidium iodide



We wish to thank Dr. Jaroslav Doležel of the Institute of Experimental Botany, Olomouc, Czech Republic for kindly providing P. sativum cv. Ctirad seeds used as internal reference standard, and Dr. Kathy Brundage of the West Virginia University Flow Cytometry Core Facility for her technical help with Flow Cytometry. The Flow Cytometry Core facility is supported by National Institutes of Health grant # RR016440, whereas this project was supported by the National Research Initiative Competitive Grant no. 2007-35318-18455 from the USDA National Institute of Food and Agriculture.


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Division of Plant and Soil SciencesWest Virginia UniversityMorgantownUSA

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