Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 102, Issue 2, pp 145–151 | Cite as

Effect of putrescine on the conversion of protocorm-like bodies of Dendrobium officinale to shoots

Original Paper
First Online:
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Accepted:

Abstract

The addition of 1.5 mM putrescine to the culture medium facilitated the conversion of protocorm-like bodies (PLBs) of Dendrobium officinale to shoots. The administration of 1.0 mM of the polyamine inhibitor, α-DL-difluoromethylarginine, decreased the conversion of PLBs to shoots. Compared to the control, the PLBs treated with 1.5 mM putrescine had higher levels of total intracellular free polyamines. The enhanced conversion of PLBs to shoots following the addition of putrescine was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA). Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by putrescine was associated with the inhibition of CTK decomposition by CTK oxidase, while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase. In addition, putrescine increased the ratio of reduced glutathione to oxidized glutathione in the conversion process of PLBs to shoots.

Keywords

Endogenous hormone Redox status Shoot development 

Abbreviations

CTKs

Cytokinins

DFMA

α-DL-difluoromethylarginine

GSH

Reduced glutathione

GSSG

Oxidized glutathione

IAA

Indole-3-acetic acid

PLBs

Protocorm-like bodies

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References

  1. Belmonte MF, Yeung EC (2004) The effects of reduced and oxidized glutathione on white spruce somatic embryogenesis. In Vitro Cell Dev Biol-Plant 40:61–66CrossRefGoogle Scholar
  2. Belmonte MF, Macey T, Yeung EC, Stasolla C (2005) The effect of osmoticum on ascorbate and glutathione metabolism during white Spruce (Picea glauca) somatic embryo development. Plant Physiol Biochem 43:337–346PubMedGoogle Scholar
  3. Carimi F, Zottini M, Formentin E, Terzi M, Lo Schiavo F (2003) Cytokinins: new apoptotic inducers in plants. Planta 216:413–421PubMedGoogle Scholar
  4. Chaoui A, Jarrar B, Ferjani EEL (2004) Effects of cadmium and copper on peroxidase, NADH oxidase and IAA oxidase activities in cell wall, soluble and microsomal membrane fraction of pea roots. J Plant Physiol 161:1225–1234CrossRefPubMedGoogle Scholar
  5. Ferreira WDM, Kerbauy GB, Kraus JE, Pescador R, Suzuki RM (2006) Thidiazuron influences the endogenous levels of cytokinins and IAA during the flowering of isolated shoots of Dendrobium. J Plant Physiol 163:1126–1134CrossRefGoogle Scholar
  6. Galston AW, Sawhney RK (1990) Polyamines in plant physiology. Plant Physiol 94:406–410CrossRefPubMedGoogle Scholar
  7. Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpridine. Anal Biochem 106:207–211CrossRefPubMedGoogle Scholar
  8. Jäger AK, Stirk WA, Staden JV (1997) Cytokinin oxidase activity in habituated and non-habituated soybean callus. Plant Growth Regul 22:203–206CrossRefGoogle Scholar
  9. Johnson TR, Stewart SL, Dutra D, Kane ME, Richardson L (2007) Asymbiotic seed germination of Eulophia alta (Orchidaceae)–preliminary evidence for the symbiotic culture advantage. Plant Cell Tissue Organ Cult 90:313–323CrossRefGoogle Scholar
  10. Liu JH, Moriguchi T (2007) Changes in free polyamine titers and expression of polymine biosynthetic genes during growth of peach in vitro callus. Plant Cell Rep 26:125–131CrossRefPubMedGoogle Scholar
  11. Luo JP, Wang Y, Zha XQ (2008) Micropropagation of Dendrobium densiflorum Lindl. ex Wall. through protocorm-like bodies: effects of plant growth regulators and lanthanoids. Plant Cell Tissue Organ Cult 93:333–340CrossRefGoogle Scholar
  12. Maldiney K, Leroux B, Sabbagh I, Sotta B, Sossountzov L, Miginiac E (1986) A biotin-avidin-based enzyme immunoassay to quantify three phytohormones: auxin, abscisic acid and zeatin riboside. J Immunol Methods 90:151–158CrossRefGoogle Scholar
  13. Martin-Tanguy J (2001) Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul 34:135–148CrossRefGoogle Scholar
  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–497Google Scholar
  15. Nayak NR, Sahoo S, Patnaik S, Rath SP (2002) Establishment of thin cross section (TCS) culture method for rapid micropropagation of Cymbidium aloifolium (L.) Sw. and Dendrobium nobile Lindl. (Orchidaceae). Sci Hortic 94:107–116CrossRefGoogle Scholar
  16. Nge KL, New N, Chandrkrachang S, Stevens WF (2006) Chitosan as a growth stimulator in orchid tissue culture. Plant Sci 170:1185–1190CrossRefGoogle Scholar
  17. Papanastaiou I, Soukouli K, Moschopoulou G, Kahia J, Kintzios S (2008) Effect of liquid pulses with 6-benzyladenine on the induction of somatic embryogenesis from coffee (Coffea arabica L.) callus cultures. Plant Cell Tissue Organ Cult 92:215–225CrossRefGoogle Scholar
  18. Roy J, Banerjee N (2003) Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. Var. oculatum HK.f. Sci Hortic 97:333–340Google Scholar
  19. Saiprasad GVS, Raghuveer P, Khetarpal S, Chandra R (2004) Effect of various polyamines on production of protocorm-like bodies in orchid Dendrobium ‘Sonia’. Sci Hortic 100:161–168CrossRefGoogle Scholar
  20. Shiau YJ, Nalawade SM, Hsia CN, Mulabagal V, Tsay HS (2005) In vitro propagation of the Chinese medicinal plant. Dendrobium candidum Wall.ex lindl., from axenic nodal segments. In Vitro Cell Dev Biol-Plant 41:666–670CrossRefGoogle Scholar
  21. Shohael AM, Ali MB, Hahn EJ, Paek KY (2007) Glutathione metabolism and antioxidant responses during Eleutherococcus senticocus somatic embryo development in a bioreactor. Plant Cell Tissue Organ Cult 89:121–129CrossRefGoogle Scholar
  22. Smith J, Ladi E, Mayer-Proschel M, Noble M (2000) Redox state is central modulator of the balance between self–renewal and differentiation in a dividing glial precursor cell. Proc Natl Acad Sci USA 97:10032–10037CrossRefPubMedGoogle Scholar
  23. Sriskandarajah S, Prinsen E, Motyka V, Dobrev PI, Serek M (2006) Regenerative capacity of Cacti Schlumbergera and Rhipsalidopsis in relation to endogenous phytohormones, cytokinin oxidase/dehydrogenase, and peroxidase activities. J Plant Growth Regul 25:79–88CrossRefGoogle Scholar
  24. Steiner N, Santa-Catarina C, Silveira VS, Floh EIS, Guerra MP (2007) Polyamine effects on growth and endogenous hormones levels in Araucaria angustifolia embryogenic cultures. Plant Cell Tissue Organ Cult 89:55–62CrossRefGoogle Scholar
  25. Sudha G, Ravishankar GA (2003) Putrescine facilitated enhancement of capsaicin production in cell suspension cultures of Capsicum frutescens. J Plant Physiol 160:339–346CrossRefPubMedGoogle Scholar
  26. Takeda T, Hayakawa F, Oe K, Matsuoka H (2002) Effects of exogenous polyamines on embryogenic carrot cells. Biochem Eng J 12:21–28CrossRefGoogle Scholar
  27. Vasudevan A, Selvaraj N, Ganapathi A, Kasthurirengan S, Anbazhagan VR, Manickavasagam M, Choi CW (2008) Leucine and spermidine enhance shoot differentiation in cucumber (Cucumis Sativus L.). In Vitro Cell Dev Biol-Plant 44:300–306CrossRefGoogle Scholar
  28. Wu XB, Wang J, Liu JH, Deng XX (2009) Involvement of polyamine biosynthesis in somatic embryogenesis of Valencia sweet orange (Citrus Sinensis) induced by glycerol. J Plant Physiol 166:52–62CrossRefPubMedGoogle Scholar
  29. Zhao PZ, Wu F, Feng FS, Wang WJ (2008) Protocorm-like bodies (PLBs) formation and plant regeneration from the callus culture of Dendrobium candidum Wall ex Lindl. In Vitro Cell Dev Biol-Plant 44:178–185CrossRefGoogle Scholar
  30. Zhu C, Chen Z (2005) Role of polyamines in adventitious shoot morphogenesis from cotyledons of cucumber in vitro. Plant Cell Tissue Organ Cult 81:45–53CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.Department of Biotechnology and ChemistryAnhui University of Technology and ScienceWuhuThe People’s Republic of China
  2. 2.Anhui Engineering Technology Research Center of Microbial FermentationWuhuThe People’s Republic of China

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