Efficient regeneration and antioxidant potential in regenerated tissues of Piper nigrum L.
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The organogenic potential and antioxidant potential (1, 1-diphenyl-2-picrylhydrazyl-scavenging activity) of the medicinal plant Piper nigrum L. (black pepper) were investigated. Callus induction and shoot regeneration were induced from leaf explants of potted plants cultured on MS medium supplemented with different plant growth regulators. The best callogenic response was observed on explants cultured for 30 days on MS medium supplemented with either 0.5 or 1.5 mg l−1 6-benzyladenine (BA) + 1.0 mg l−1 α-naphthaleneacetic acid. Subsequent transfer of the callogenic explants onto MS medium supplemented with 1.5 mg l−1 BA + 1.0 mg l−1 gibberellic acid (GA3) achieved 85% shoot organogenesis after 30 days of culture. The maximum number (7.2) of shoots/explant was recorded for explants cultured in MS medium supplemented with 1.0 mg l−1 BA. Following the transfer of shoots to an elongation medium, the longest shoots (5.4 cm) were observed on MS medium supplemented with 1.0 mg l−1 BA + 1.0 mg l−1 GA3. The elongated shoots were rooted on MS medium supplemented with different concentrations of indole butyric acid. An assay of the antioxidant potential of the in vitro-grown tissues revealed that the antioxidant activity of the regenerated shoots was significantly higher than that of callus and the regenerated plantlets.
KeywordsAntioxidant 6-Benzyladenine Gibberellic acid Organogenesis Piper nigrum
- 2,4 D
2,4 Dichlorophenoxyacetic acid
Indole butyric acid
MS medium without plant growth regulators
Plant growth regulators
The authors acknowledge the support of Higher Education Commission (HEC) of Pakistan and University Research Fund (URF) of Quaid-i-Azam University (QAU), Islamabad, Pakistan.
- Abbasi BH, Khan MA, Mahmood T, Ahmad M, Chaudhary MF, Khan MA (2010a) Shoot regeneration and free-radical scavenging activity in Silybum marianum L. Plant Cell Tissue Org Cult. doi 10.1007/s11240-010-9692-x
- Abbasi BH, Ahmad N, Fazal H, Mahmood T (2010b) Conventional and modern propagation techniques in Piper nigrum. J Med Plant Res 4:007–012Google Scholar
- Atak C, Celik O (2009) Micropropagation of Anthurium andraeanum from leaf explants. Pak J Bot 41:1155–1161Google Scholar
- Eeswara JP, Allan EJ, Mordue J, Stuchbury T (1999) A procedure for sterilizing leaf explants collected from wild neem (Azadirachta indica) trees. J Natl Sci Found Sri Lanka 27:131–136Google Scholar
- Kelkar SM, Deboo GB, Krishnamurthy KV (1996) In vitro plant regeneration from leaf callus in Piper colubrinum link. Plant Cell Rep 14:398–402Google Scholar
- Lee SA, Hong SS, Han XB, Hwang JS, Oh GJ, Lee KS, Lee MK, Hwang BY, Ro JS (2005) Piperine from the fruits of Piper nigrum with inhibitory effect on monoamine oxidase and antidepressant like activity. Chem Pharm Bull 53:832–835Google Scholar
- Lucchesinia M, Bertolib A, Mensuali-Sodic A, Pistelli L (2009) Establishment of in vitro tissue cultures from Echinacea angustifolia D.C. adult plants for the production of phytochemical compounds. Sci Hortic 122:484–490Google Scholar
- Magyar-Tabori K, Dobranszki J, da Silva JAT, Belley SM, Hudak I (2010) The role of cytokinins in shoot organogenesis in apple. Plant Cell Tiss Organ Cult. doi 10.1007/s11240-010-9696-6
- Rubluo M, Barroso M (1992). In vitro morphogenetic responses and cytokinin-auxin interaction for callus production in pepper. An Inst Biol Univ Nacional Autonoma Mexico Ser Bot 63(2):195–201Google Scholar
- Shilpa K, Selvakkumar C, Senthil AK, Lakshmi BS (2010) In vitro root culture of Ocimum sanctum L. and evaluation of its free radical scavenging activity. Plant Cell Tiss Org Cult. doi 10.1007/s11240-009-9661-4