Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 101, Issue 3, pp 295–302 | Cite as

Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation

  • Hong Soon Rhee
  • Hwa-Young Cho
  • Seok Young Son
  • Sung-Yong H. Yoon
  • Jong Moon Park
Original Paper


Angelica gigas root cultures were elicited with various elicitors, including yeast extract, chitin, methyl jasmonate, salicylic acid, and copper, with the aim of increasing the production of decursin and decursinol angelate. The treatment of A. gigas root cultures with a combination of yeast extract (2 g l−1) and copper ion (0.5 mM) at the late exponential growth phase increased decursinol angelate accumulation up to 6.86 mg l−1. The best elicitor preparation selected through in vitro experiments was also applied to roots of A. gigas whole plants grown in the field in order to investigate the potential of elicitation as a novel cultivation practice for producing medicinal herbs of improved quality. Biweekly treatments with the elicitor at 70 mg g l−1 FW roots for 10 weeks before the annual harvest resulted in an increment in both plant yields and specific productivity of decursins by 1.5- and 1.7-fold, respectively. This result implies that in vitro screening of elicitors with the ultimate aim of in planta elicitation of whole plants could be effective in terms of time and expense. The elicitation technique reported here demonstrates it potential as a strategy for improving growth and active compounds productivity of medicinal plants through short-term and pre-harvest treatment of the elicitor preparation.


Angelica gigas Nakai Copper ion Decursin Decursinol angelate Elicitor preparation Plant root culture in planta elicitation Yeast extract 



Chitin [poly(N-acetyl-14-β-d-glucosamine]


Decursinol angelate




Indole-3-butyric acid


Methyl jasmonate


Salicylic acid


Yeast extract



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant number 2009-0079288).


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hong Soon Rhee
    • 1
  • Hwa-Young Cho
    • 2
  • Seok Young Son
    • 1
  • Sung-Yong H. Yoon
    • 3
  • Jong Moon Park
    • 1
  1. 1.Advanced Environmental Biotechnology Research Center, Department of Chemical Engineering, School of Environmental Science and EngineeringPohang University of Science and TechnologyPohangSouth Korea
  2. 2.Samsung Advanced Institute of TechnologyYonginSouth Korea
  3. 3.Exelixis Plant SciencesPortlandUSA

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