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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 101, Issue 3, pp 295–302 | Cite as

Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation

  • Hong Soon Rhee
  • Hwa-Young Cho
  • Seok Young Son
  • Sung-Yong H. Yoon
  • Jong Moon Park
Original Paper

Abstract

Angelica gigas root cultures were elicited with various elicitors, including yeast extract, chitin, methyl jasmonate, salicylic acid, and copper, with the aim of increasing the production of decursin and decursinol angelate. The treatment of A. gigas root cultures with a combination of yeast extract (2 g l−1) and copper ion (0.5 mM) at the late exponential growth phase increased decursinol angelate accumulation up to 6.86 mg l−1. The best elicitor preparation selected through in vitro experiments was also applied to roots of A. gigas whole plants grown in the field in order to investigate the potential of elicitation as a novel cultivation practice for producing medicinal herbs of improved quality. Biweekly treatments with the elicitor at 70 mg g l−1 FW roots for 10 weeks before the annual harvest resulted in an increment in both plant yields and specific productivity of decursins by 1.5- and 1.7-fold, respectively. This result implies that in vitro screening of elicitors with the ultimate aim of in planta elicitation of whole plants could be effective in terms of time and expense. The elicitation technique reported here demonstrates it potential as a strategy for improving growth and active compounds productivity of medicinal plants through short-term and pre-harvest treatment of the elicitor preparation.

Keywords

Angelica gigas Nakai Copper ion Decursin Decursinol angelate Elicitor preparation Plant root culture in planta elicitation Yeast extract 

Abbreviations

CH

Chitin [poly(N-acetyl-14-β-d-glucosamine]

DA

Decursinol angelate

DC

Decursin

IBA

Indole-3-butyric acid

MJ

Methyl jasmonate

SA

Salicylic acid

YE

Yeast extract

Notes

Acknowledgments

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant number 2009-0079288).

References

  1. Ahn KS, Sim WS, Kim IH (1996) Decursin: a cytotoxic agent and protein kinase C activator from the root of Angelica gigas. Planta Med 62:7–9. doi: 10.1055/s-2006-957785 CrossRefPubMedGoogle Scholar
  2. Aziz A, Trotel-Aziz P, Dhuicq L, Jeandet P, Couderchet M, Vernet G (2006) Chitosan oligomers and copper sulfate induce grapevine defense reactions and resistance to gray mold and downy mildew. Phytopathology 96:1188–1194. doi: 10.1094/PHYTO-96-1188 CrossRefPubMedGoogle Scholar
  3. Bais HP, Govindaswamy S, Ravishankar GA (2000) Enhancement of growth and coumarin production in hairy root cultures of witlof chicory (Cichorium intybus L. cv. Lucknow local) under the influence of fungal elicitors. J Biosci Bioeng 90:648–653. doi: 10.1016/S1389-1723(00)90011-2 CrossRefPubMedGoogle Scholar
  4. Bautista-Baños S, Hernández-Lauzardo AN, Velázquez-Del Valle MG, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson CL (2006) Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Prot 25:108–118. doi: 10.1016/j.cropro.2005.03.010 CrossRefGoogle Scholar
  5. Bhuiyan Md NH, Adachi T (2003) Stimulation of betacyanin synthesis through exogenous methyl jasmonate and other elicitors in suspension-cultured cells of Portulaca. J Plant Physiol 160:1117–1124. doi: 10.1078/0176-1617-01044 CrossRefGoogle Scholar
  6. Boller T (1995) Chemoperception of microbial signals in plant cells. Annu Rev Plant Physiol Plant Mol Biol 46:189–214. doi: 10.1146/annurev.pp.46.060195.001201 CrossRefGoogle Scholar
  7. Cho JS, Kim JY, Kim IH, Kim DI (2003) Effect of polysaccharide elicitors on the production of decursinol angelate in Angelica gigas Nakai root cultures. Biotechnol Bioprocess Eng 8:158–161. doi: 10.1007/BF02940273 CrossRefGoogle Scholar
  8. Cho HY, Rhee HS, Yoon SYH, Park JM (2008a) Differential induction of protein expression and benzophenanthridine alkaloid accumulation in Eschscholtzia californica suspension cultures by methyl jasmonate and yeast extract. J Microbiol Biotechnol 18:255–262PubMedGoogle Scholar
  9. Cho HY, Son SY, Rhee HS, Yoon SYH, Lee-Parsons CWT, Park JM (2008b) Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures. J Biotechnol 135:117–122. doi: 10.1016/j.jbiotec.2008.02.020 CrossRefPubMedGoogle Scholar
  10. Choi HK, Yun JH, Kim SI, Son JS, Kim HR, Kim JH, Choi HJ, Hong SS (2001) Enhanced production of paclitaxel by semi-continuous batch process (SCBP) in suspension culture of Taxus chinensis. Enzyme Microb Technol 29:583–586. doi: 10.1016/S0141-0229(01)00427-6 CrossRefGoogle Scholar
  11. Dicke M, Dijkman H (2001) Within-plant circulation of systemic elicitor of induced defence and release from roots of elicitor that affects neighbouring plants. Biochem Syst Ecol 29:1075–1087. doi: 10.1016/S0305-1978(01)00051-5 CrossRefGoogle Scholar
  12. Fliegmann J, Schüler G, Boland W, Ebel J, Mithöfer A (2003) The role of octadecanoids and functional mimics in soybean defense responses. Biol Chem 384:437–446. doi: 10.1515/BC.2003.049,March2003 CrossRefPubMedGoogle Scholar
  13. Gomez-Galera S, Pelacho AM (2007) The genetic manipulation of medicinal and aromatic plants. Plant Cell Rep 26:1689–1715. doi: 10.1007/s00299-007-0384-x CrossRefPubMedGoogle Scholar
  14. Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci USA 89:2289–2293CrossRefGoogle Scholar
  15. Gutiérrez-Coronado MA, Parry A, Tena M, Jorrin J, Edwards R (1995) Abiotic elicitation of coumarin phytoalexins in sunflower. Phytochemistry 38:1185–1191. doi: 10.1016/0031-9422(94)00808-7 CrossRefGoogle Scholar
  16. Gutiérrez-Coronado MA, Trejo-López C, Larqué-Saavedra A (1998) Effect of salicylic acid on the growth of roots and shoots in soybean. Plant Physiol Biochem 36:563–565. doi: 10.1016/S0981-9428(98)80003-X CrossRefGoogle Scholar
  17. Hahn MG, Albersheim P (1978) Host-pathogen interactions. XIV. Isolation and partial characterization of elicitor from yeast extract. Plant Physiol 62:107–111CrossRefPubMedGoogle Scholar
  18. Kang SY, Lee KY, Sung SH, Park MJ, Kim YC (2001) Coumarins isolated from Angelica gigas inhibit acetylcholinesterase: Structure-activity relationships. J Nat Prod 64:683–685. doi: 10.1021/np000441w CrossRefPubMedGoogle Scholar
  19. Kang SY, Lee KY, Park MJ, Kim YC, Markelonis GJ, Oh TH, Kim YC (2003) Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol Learn Mem 79:11–18. doi: 10.1016/S1074-7427(02)00007-2 CrossRefPubMedGoogle Scholar
  20. Kap JP, Yang S, Young AE, Soo YK, Hyung HL, Kang H (2002) Cytotoxic effects of Korean medicinal herbs determined with hepatocellular carcinoma cell lines. Pharm Biol 40:189–195. doi: 10.1076/phbi.40.3.189.5824 CrossRefGoogle Scholar
  21. Kim JY, Cho JS, Moon IH (2002) Effect of root segmentation and plant growth regulator on decursinol angelate production in Angelica gigas Nakai. Korean J Biotechnol Bioeng 17:461Google Scholar
  22. Kim MR, El-Aty AMA, Kim IS, Shim JH (2006) Determination of volatile flavor components in danggui cultivars by solvent free injection and hydrodistillation followed by gas chromatographic-mass spectrometric analysis. J Chromatogr A 1116:259–264. doi: 10.1016/j.chroma.2006.03.060 CrossRefPubMedGoogle Scholar
  23. Kombrink E, Hahlbrock K (1986) Responses of cultured parsley cells to elicitors from phytopathogenic fungi. Plant Physiol 81:216–221. doi: 0032-0889/86/81/0216/06$01.00/0 CrossRefPubMedGoogle Scholar
  24. Lafontaine PJ, Benhamou N (1996) Chitosan treatment: an emerging strategy for enhancing resistance of greenhouse tomato plants to infection by Fusarium oxysporum f.sp. radicis-lycopersici. Biocontrol Sci Tech 6:111–124. doi: 10.1080/09583159650039575 CrossRefGoogle Scholar
  25. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497Google Scholar
  26. Ryu CM, Murphy JF, Reddy MS, Kloepper JW (2007) A two-strain mixture of rhizobacteria elicits induction of systemic resistance against Pseudomonas syringae and Cucumber mosaic virus coupled to promotion of plant growth on Arabidopsis thaliana. J Microbiol Biotechnol 17:280–286PubMedGoogle Scholar
  27. Schützendübel A, Polle A (2002) Plant responses to abiotic stresses: heavy metal-induced oxidative stress and protection by mycorrhization. J Exp Bot 53:1351–1365CrossRefPubMedGoogle Scholar
  28. Siatka T, Kasparova M (2007) Effect of vanadium compounds on the growth and production of coumarins in the suspension culture of Angelica archangelica L. Ceska Slovenska Farm 56:230–234Google Scholar
  29. Tabata M, Fujita Y (1985) Production of shikonin by plant cell cultures. In: Day P, Zaitlin M, Hollaender A (eds) Biotechnology in plant science. Academic Press, Orlando, pp 207–218Google Scholar
  30. Thaler JS, Fidantsef AL, Duffey SS, Bostock RM (1999) Trade-offs in plant defense against pathogens and herbivores: a field demonstration of chemical elicitors of induced resistance. J Chem Ecol 25:1597–1609. doi: 10.1023/A:1020840900595 CrossRefGoogle Scholar
  31. Yim D, Singh RP, Agarwal C, Lee S, Chi H, Agarwal R (2005) A novel anticancer agent, decursin, induces G1 arrest and apoptosis in human prostate carcinoma cells. Cancer Res 65:1035–1044CrossRefPubMedGoogle Scholar
  32. Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333. doi: 10.1016/j.biotechadv.2005.01.003 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hong Soon Rhee
    • 1
  • Hwa-Young Cho
    • 2
  • Seok Young Son
    • 1
  • Sung-Yong H. Yoon
    • 3
  • Jong Moon Park
    • 1
  1. 1.Advanced Environmental Biotechnology Research Center, Department of Chemical Engineering, School of Environmental Science and EngineeringPohang University of Science and TechnologyPohangSouth Korea
  2. 2.Samsung Advanced Institute of TechnologyYonginSouth Korea
  3. 3.Exelixis Plant SciencesPortlandUSA

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