Enhanced accumulation of decursin and decursinol angelate in root cultures and intact roots of Angelica gigas Nakai following elicitation
- 459 Downloads
Angelica gigas root cultures were elicited with various elicitors, including yeast extract, chitin, methyl jasmonate, salicylic acid, and copper, with the aim of increasing the production of decursin and decursinol angelate. The treatment of A. gigas root cultures with a combination of yeast extract (2 g l−1) and copper ion (0.5 mM) at the late exponential growth phase increased decursinol angelate accumulation up to 6.86 mg l−1. The best elicitor preparation selected through in vitro experiments was also applied to roots of A. gigas whole plants grown in the field in order to investigate the potential of elicitation as a novel cultivation practice for producing medicinal herbs of improved quality. Biweekly treatments with the elicitor at 70 mg g l−1 FW roots for 10 weeks before the annual harvest resulted in an increment in both plant yields and specific productivity of decursins by 1.5- and 1.7-fold, respectively. This result implies that in vitro screening of elicitors with the ultimate aim of in planta elicitation of whole plants could be effective in terms of time and expense. The elicitation technique reported here demonstrates it potential as a strategy for improving growth and active compounds productivity of medicinal plants through short-term and pre-harvest treatment of the elicitor preparation.
KeywordsAngelica gigas Nakai Copper ion Decursin Decursinol angelate Elicitor preparation Plant root culture in planta elicitation Yeast extract
This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (Grant number 2009-0079288).
- Bais HP, Govindaswamy S, Ravishankar GA (2000) Enhancement of growth and coumarin production in hairy root cultures of witlof chicory (Cichorium intybus L. cv. Lucknow local) under the influence of fungal elicitors. J Biosci Bioeng 90:648–653. doi: 10.1016/S1389-1723(00)90011-2 CrossRefPubMedGoogle Scholar
- Bautista-Baños S, Hernández-Lauzardo AN, Velázquez-Del Valle MG, Hernández-López M, Ait Barka E, Bosquez-Molina E, Wilson CL (2006) Chitosan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Prot 25:108–118. doi: 10.1016/j.cropro.2005.03.010 CrossRefGoogle Scholar
- Cho HY, Son SY, Rhee HS, Yoon SYH, Lee-Parsons CWT, Park JM (2008b) Synergistic effects of sequential treatment with methyl jasmonate, salicylic acid and yeast extract on benzophenanthridine alkaloid accumulation and protein expression in Eschscholtzia californica suspension cultures. J Biotechnol 135:117–122. doi: 10.1016/j.jbiotec.2008.02.020 CrossRefPubMedGoogle Scholar
- Kim JY, Cho JS, Moon IH (2002) Effect of root segmentation and plant growth regulator on decursinol angelate production in Angelica gigas Nakai. Korean J Biotechnol Bioeng 17:461Google Scholar
- Kim MR, El-Aty AMA, Kim IS, Shim JH (2006) Determination of volatile flavor components in danggui cultivars by solvent free injection and hydrodistillation followed by gas chromatographic-mass spectrometric analysis. J Chromatogr A 1116:259–264. doi: 10.1016/j.chroma.2006.03.060 CrossRefPubMedGoogle Scholar
- Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15(3):473–497Google Scholar
- Siatka T, Kasparova M (2007) Effect of vanadium compounds on the growth and production of coumarins in the suspension culture of Angelica archangelica L. Ceska Slovenska Farm 56:230–234Google Scholar
- Tabata M, Fujita Y (1985) Production of shikonin by plant cell cultures. In: Day P, Zaitlin M, Hollaender A (eds) Biotechnology in plant science. Academic Press, Orlando, pp 207–218Google Scholar