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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 101, Issue 1, pp 99–103 | Cite as

Production of bakuchiol by in vitro systems of Psoralea drupacea Bge

  • Kateryna LystvanEmail author
  • Valeria Belokurova
  • Yuriy Sheludko
  • John L. Ingham
  • Valeria Prykhodko
  • Olena Kishchenko
  • Evgenija Paton
  • Mykola Kuchuk
Research Note

Abstract

In vitro production of the meroterpene bakuchiol by Psoralea drupacea Bge (Fabaceae) has been studied using aseptically-grown plants, callus cultures of different origin, cell suspensions and transgenic hairy root cultures. The effect of phytohormones and methyl jasmonate on bakuchiol production was also investigated. Bakuchiol was not detected in cell suspensions or hairy root preparations of P. drupacea. In contrast, aerial parts of P. drupacea grown in vitro were found to accumulate up to 11% dry weight of bakuchiol and can therefore be regarded as a potentially useful source of this antimicrobial compound.

Keywords

Bakuchiol Psoralea Psoralea drupacea In vitro cultures Antimicrobial Secondary metabolite 

Abbreviations

BA

6-Benzyladenine

HPLC

High-performance liquid chromatography

kin

Kinetin

LB

Luria-Bertani medium

MIC

Minimum inhibitory concentration

MS

Murashige and Skoog medium

NAA

1-Naphthaleneacetic acid

TDZ

Thidiazuron

2,4-D

2,4-Dichlorophenoxyacetic acid

Notes

Acknowledgments

This work is part of the project “Establishment and Application of Cell Cultures—Producers of Plant Antimicrobial and Antifungal Substances” (0107U004770) supported by the National Academy of Science of Ukraine.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Kateryna Lystvan
    • 1
    Email author
  • Valeria Belokurova
    • 1
  • Yuriy Sheludko
    • 1
  • John L. Ingham
    • 2
  • Valeria Prykhodko
    • 3
  • Olena Kishchenko
    • 1
  • Evgenija Paton
    • 1
  • Mykola Kuchuk
    • 1
  1. 1.Institute of Cell Biology and Genetic EngineeringNational Academy of Sciences of UkraineKievUkraine
  2. 2.Plantech (UK)ReadingUK
  3. 3.Institute of Microbiology and VirologyNational Academy of Sciences of UkraineKievUkraine

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