Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Shoot regeneration from leaf explants of Brunfelsia calycina

  • 239 Accesses

  • 4 Citations


A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23–2.46 μM indole butrytic acid (IBA), they developed roots.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4









Indole-3-acetic acid


Indole-3-butyric acid


Murashige and Skoog (1962)


2-Naphthaleneacetic acid


Thidiazuron (N-phenyl N′ 1,2,3-thidiazol-5-yl urea)


  1. Brenzel KN (2001) Western garden book, 7th edn. Sunset Publishing Corporation, Menlo Park

  2. Halevy AH (1985) Handbook of flowering. CRC Press, Boca Raton

  3. Heide OM (1963) Effect of temperature and day-length on flower initiation in Brunfelsia calycina (Hook) Benth. Physiol Plant 16:104–107

  4. Mohamed AA, Aly AA (2004) Iron deficiency stimulated enzyme activity, lipid peroxidation and free radical production in Borago officinalis induced in vitro. Int J Agri Biol 6:179–184

  5. Murashige T, Skoog F (1962) A medium for rapid growth and bio-assay with tobacco tissue culture. Physiol Plant 15:437–497

  6. Plowman T (1977) Brunfelsia in ethnomedicine. Bot Mus Leafl (Harvard Univ) 25:289–320

  7. Reuveni M, Evenor D, Ben-Jaacov J (2003) On the road to micro-propagation of Banksia ashbyi. In: Proceedings of 2002 international protea meeting. Acta Hort 602:149–154

  8. Reuveni M, Evenor D, Kagan S, Ben-Jaacov J (2005) Rejuvenation by in vitro propagation from mature Hermannia stricta (Sterculiaceae) plants. Int J Appl Agri Res 1(1):20–26

  9. Vaknin H, Bar-Akiva A, Ovadia R, Nissim-Levi A, Forer I, Weiss D, Oren-Shamir M (2005) Active anthocyanin degradation in Brunfelsia calycina (Yesterday–Today–Tomorrow) flowers. Planta 222:19–26

Download references

Author information

Correspondence to Michal Oren-Shamir.

Additional information

Raya Liberman and Liat Shahar have equally contributed to this work.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Liberman, R., Shahar, L., Nissim-Levi, A. et al. Shoot regeneration from leaf explants of Brunfelsia calycina . Plant Cell Tiss Organ Cult 100, 345–348 (2010). https://doi.org/10.1007/s11240-009-9642-7

Download citation


  • Regeneration
  • Solanaceae
  • Brunfelsia