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Shoot regeneration from leaf explants of Brunfelsia calycina

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Abstract

A protocol for plantlet regeneration through shoot formation was developed for the neotropical shrub Brunfelsia calycina. This shrub is unique in its change in flower color from dark purple to white. Explants from young and mature leaves were incubated on MS medium (pH 5.7, 30 g/l sucrose, 7.5 g/l agar) with various combinations of Indole-3-acetic acid (IAA) and 6-Benzyladenine (BA) under a 16 h photoperiod at a constant temperature of 25°C. Shoot emergence was best at 4.44 μM BA and 2.85 μM IAA for young leaf explants, and at 8.88 μM BA, 2.85 μM IAA for mature leaf explants. When shoots were transferred to MS medium supplemented with 1.23–2.46 μM indole butrytic acid (IBA), they developed roots.

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Abbreviations

Aux:

Auxin

BA:

6-Benzyladenine

Ck:

Cytokinin

IAA:

Indole-3-acetic acid

IBA:

Indole-3-butyric acid

MS:

Murashige and Skoog (1962)

NAA:

2-Naphthaleneacetic acid

TDZ:

Thidiazuron (N-phenyl N′ 1,2,3-thidiazol-5-yl urea)

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Author information

Correspondence to Michal Oren-Shamir.

Additional information

Raya Liberman and Liat Shahar have equally contributed to this work.

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Liberman, R., Shahar, L., Nissim-Levi, A. et al. Shoot regeneration from leaf explants of Brunfelsia calycina . Plant Cell Tiss Organ Cult 100, 345–348 (2010). https://doi.org/10.1007/s11240-009-9642-7

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Keywords

  • Regeneration
  • Solanaceae
  • Brunfelsia