Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 100, Issue 2, pp 149–156 | Cite as

Genetic transformation of the oilseed crop Crambe abyssinica

  • Xueyuan Li
  • Annelie Ahlman
  • Xiaofeng Yan
  • Helén Lindgren
  • Li-Hua Zhu
Original Paper

Abstract

Engineering oilseed crops for industrial purposes requires a suitable crop that does not outcross to any food oilseed crop, thus eliminating problems of gene flow. Crambe abyssinica is such a dedicated crop as it does not hybridize with any existing food oilseed crops. However, lack of regeneration and transformation protocols has limited the use of C. abyssinica in genetic manipulation studies. In this study, efficient regeneration and transformation protocols for Crambe have been developed. Hypocotyls of C. abyssinica cv. Galactica were incubated on a Murashige and Skoog medium supplemented with various plant growth regulators (PGRs). Among the different PGR combinations tested, 10 μM thidiazuron and 2.7 μM α-naphthaleneacetic acid promoted highest frequency of regeneration, up to 60%. Among six Agrobacterium stains evaluated, each harbouring the cloning vector containing the neomycin phosphotransferase (nptII) and β-glucuronidase (gus) genes. EHA101 and AGL-1 yielded the highest transformation frequencies of 1.3 and 2.1%, respectively. Putative transgenic lines were recovered, and confirmed as transgenic by Southern blot analysis. Subsequently, Agrobacterium-mediated transformation of hypocotyls of cv. Galactica with constructs harbouring the wax synthase and fatty acid reductase genes have also successfully recovered confirmed transgenic plants carrying these transgenes.

Keywords

Crambe abyssinica Regeneration Agrobacterium transformation 

Abbreviations

BA

6-Benzyladnine

GA

Gibberellin

GUS

β-Glucuronidase

IBA

3-Indole-butyric acid

NAA

α-Naphthaleneacetic acid

nptII

Neymycin phosphotransferase

TDZ

Thidiazuron

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Xueyuan Li
    • 1
  • Annelie Ahlman
    • 1
  • Xiaofeng Yan
    • 1
    • 2
  • Helén Lindgren
    • 1
  • Li-Hua Zhu
    • 1
  1. 1.Department of Plant Breeding and BiotechnologySwedish University of Agricultural ScienceAlnarpSweden
  2. 2.Hubei UniversityWuhanChina

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