Transgenic rice was developed from ‘Swarna’, the most popular indica rice cultivar (Oryza sativa L.) in South East Asia, with a potato chymotrypsin inhibitor gene (pin2) through Agrobacterium-mediated transformation. Four out of nine primary transgenic plants had a single-copy T-DNA insertion while other five plants had two copies. Mendelian pattern of inheritance of the transgene (pin2) was observed in the T1 generation progeny plants. Whole plant bioassays conducted at both vegetative and reproductive stages and cut stem assays showed enhanced levels of resistance of transgenic rice against yellow stem borer. The transgenic rice lines with plant derived proteinase inhibitor genes would develop into resistant cultivars to fit into resistance breeding strategies as an important component of integrated pest management in rice.
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We thank Dr Richard A. Jefferson, CAMBIA, Australia for providing pCAMBIA1301 and Dr. K. Veluthambi, Madurai Kamaraj University, India for Agrobacterium strains. We thank Prof. Clarence A. Ryan, Washington State University, USA for gifting us the pin2 gene. MVR, KSB, and GJNR are thankful to the Director, CRRI and Head, Division of Crop Improvement, CRRI for the laboratory and field facilities used in the research. This work was supported by the Rockefeller foundation and IRRI in the form of a grant (ICAR/IRRI/CRRI collaborative project grant) and a senior research fellowship to MVR.
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Rao, M.V.R., Behera, K.S., Baisakh, N. et al. Transgenic indica rice cultivar ‘Swarna’ expressing a potato chymotrypsin inhibitor pin2 gene show enhanced levels of resistance to yellow stem borer. Plant Cell Tiss Organ Cult 99, 277–285 (2009). https://doi.org/10.1007/s11240-009-9602-2
- Indica rice
- Proteinase inhibitor