Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 99, Issue 2, pp 151–156

Cardiotonic glycosides from biomass of Digitalis purpurea L. cultured in temporary immersion systems

  • Naivy Pérez-Alonso
  • Dirk Wilken
  • André Gerth
  • Annett Jähn
  • Horst-Michael Nitzsche
  • Gerhard Kerns
  • Alina Capote-Perez
  • Elio Jiménez
Original Paper
  • 484 Downloads

Abstract

Cardiotonic glycosides are extracted mostly from leaves of Digitalis plants. Commercial production of bioactive secondary metabolites by traditional agriculture is an inefficient process and can be affected by climatic and soil conditions. Strategies, based on in vitro culture methods, have been extensively studied to improve the production of specific plant derived chemicals. The aim of the present research was to obtain biomass of D. purpurea using the temporary immersion system (TIS) and to determine the content of cardiotonic glycosides (digitoxin, digoxin and lanatoside C) as secondary metabolites of commercial value for the pharmaceutical industry. Shoots were cultured in 1,000 ml TIS during 28 days. The effect of four immersion frequencies (once every 2, 4, 6, and 12 h) was studied. Biomass accumulation was influenced by immersion frequency. The maximum biomass accumulation (values in respect of fresh and dry weight) was obtained with immersions every 4 h (six immersions per day). HPLC analysis revealed the presence of digoxin and digitoxin for all immersion frequencies. No lanatoside C was detected in the biomass cultured in TIS. Digoxin concentrations varied depending on the frequencies tested. In contrast, the digitoxin content showed no dependency on the immersion frequency. Net production of digoxin and digitoxin per TIS were found to be higher with immersions every 4 h. The best net production of digitoxin and digoxin per TIS were 167.6 and 119.9 μg, respectively. The development of organ culture based on temporary immersion system can be a reliable method for the steady production of biomass for cardiotonic glycosides production, which is reported for the first time for Digitalis genus in this communication.

Keywords

Digitoxin Digoxin In vitro culture Liquid medium Plant secondary metabolites 

Abbreviations

6-BAP

6-Benzylaminopurine

DW

Dry weight

FW

Fresh weight

IAA

Indole-3-acetic acid

TIS

Temporary immersion system

MS

Murashige and Skoog medium

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Naivy Pérez-Alonso
    • 1
  • Dirk Wilken
    • 2
  • André Gerth
    • 2
  • Annett Jähn
    • 3
  • Horst-Michael Nitzsche
    • 3
  • Gerhard Kerns
    • 4
  • Alina Capote-Perez
    • 1
  • Elio Jiménez
    • 1
  1. 1.Instituto de Biotecnología de las PlantasUniversidad Central “Marta Abreu” de Las VillasSanta ClaraCuba
  2. 2.BioPlanta GmbHLeipzigGermany
  3. 3.Institute of Non-Classic ChemistryLeipzig University (INC)LeipzigGermany
  4. 4.Saxon Institute for Applied BiotechnologyLeipzig UniversityLeipzigGermany

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