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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 99, Issue 1, pp 73–81 | Cite as

Effect of plant growth regulators on ovary culture of coconut (Cocos nucifera L.)

  • P. I. P. PereraEmail author
  • V. R. M. Vidhanaarachchi
  • T. R. Gunathilake
  • D. M. D. Yakandawala
  • V. Hocher
  • J. L. Verdeil
  • L. K. Weerakoon
Original Paper

Abstract

Coconut is a cross pollinating palm, propagated only by seeds. Tissue culture is the only vegetative propagation method available for coconut. Consistent callogenesis was obtained by culturing unfertilised ovaries at -4 stage in CRI 72 medium containing 100 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.1% activated charcoal. Callusing was improved by application of 9 μM thidiazuron (TDZ). Embryogenic calli were subcultured onto somatic embryogenesis induction medium containing 66 μM 2,4-D. Stunted growth was observed in the somatic embryos after subculture onto CRI 72 medium containing abscisic acid (ABA). Maturation of somatic embryos could be achieved in Y3 medium without growth regulators. Conversion of somatic embryos was induced by adding gibberellic acid (GA3) to conversion medium containing 5 μM 6-benzyladenine (BA) while 2-isopentyl adenine (2iP) increased the frequency of plant regeneration. A total of 83 plantlets was produced from 32 cultured ovaries.

Keywords

Callogenesis Regeneration Somatic embryogenesis Palm Arecaceae 

Abbreviations

ABA

Abscisic acid

2,4-D

2,4-Dichlorophenoxyacetic acid

BA

6-Benzyladenine

2iP

2-Isopentyl adenine

GA3

Gibberellic acid

TDZ

Thidiazuron

Notes

Acknowledgments

We are thankful to J.D.J.S. Kularatne, Biometry Division, Coconut Research Institute, Lunuwila, Sri Lanka for his assistance in statistical analysis. Special thanks go to Mr. Kapila Prasanna, Tissue Culture Division, Coconut Research Institute, Sri Lanka, for careful attention in sample collection.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • P. I. P. Perera
    • 1
    • 5
    Email author
  • V. R. M. Vidhanaarachchi
    • 1
  • T. R. Gunathilake
    • 1
  • D. M. D. Yakandawala
    • 2
    • 5
  • V. Hocher
    • 3
  • J. L. Verdeil
    • 4
  • L. K. Weerakoon
    • 1
  1. 1.Tissue Culture DivisionCoconut Research InstituteLunuwilaSri Lanka
  2. 2.Department of BotanyUniversity of PeradeniyaPeradeniyaSri Lanka
  3. 3.Institute for Research and Development (IRD)UMR 1098 BEPC, IRDMontpellier Cedex 1France
  4. 4.CIRADMontpellier Cedex 5France
  5. 5.Postgraduate Institute of ScienceUniversity of PeradeniyaPeradeniyaSri Lanka

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