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Plant Cell, Tissue and Organ Culture (PCTOC)

, Volume 99, Issue 1, pp 61–71 | Cite as

Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)

  • Pradeep C. Deo
  • Robert M. Harding
  • Mary Taylor
  • Anand P. Tyagi
  • Douglas K. Becker
Original Paper

Abstract

Callus was initiated in three different “esculenta” taro cultivars by culturing corm slices in the dark on half-strength MS medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by subculture of all corm slices to half-strength MS medium containing 1.0 mg/l thidiazuron (TDZ). Depending on the cultivar, 20–30% of corm slices produced compact, yellow, nodular callus on media containing TDZ. Histological studies revealed the presence of typical embryogenic cells which were small, isodiametric with dense cytoplasms. Somatic embryos formed when callus was transferred to hormone-free medium and ~72% of the embryos germinated into plantlets on this medium. Simultaneous formation of roots and shoots during germination, and the presence of shoot and root poles revealed by histology, confirmed that these structures were true somatic embryos. Plants derived from somatic embryos appeared phenotypically normal following 2 months growth in a glasshouse. This method is a significant advance on those previously reported for the esculenta cultivars of taro due to its efficiency and reproducibility.

Keywords

Somatic embryogenesis Callus Taro TDZ Colocasia esculenta var. esculenta 

Notes

Acknowledgments

The authors wish to thank New Zealand’s International Aid and Development Agency, University Research Committee-The University of the South Pacific, Faculty of Science, Technology and Environment-The University of the South Pacific for all financial assistance and Centre for Tropical Crops and Biocommodities-Queensland University of Technology, Secretariat of the Pacific Community and The University of the South Pacific for technical support during this project. PCD was a PhD candidate at The University of the South Pacific.

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Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Pradeep C. Deo
    • 1
    • 2
  • Robert M. Harding
    • 2
  • Mary Taylor
    • 3
  • Anand P. Tyagi
    • 1
  • Douglas K. Becker
    • 2
  1. 1.School of Biological and Chemical Sciences, Faculty of Science, Technology and EnvironmentUniversity of the South PacificSuvaFiji
  2. 2.Centre for Tropical Crops and Biocommodities, Faculty of ScienceQueensland University of TechnologyBrisbaneAustralia
  3. 3.Centre for Pacific Crops and TreesSecretariat of the Pacific CommunitySuvaFiji

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