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Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

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Abstract

The influence of the developmental stage of microspores on establishing isolated microspore cultures of three Hungarian (‘Szegedi 80’, ‘Szegedi 178’, and ‘Remény’) and three Spanish (‘Jeromin’, ‘Jariza’, and ‘Jaranda’) pepper genotypes was investigated. Donor anthers containing 80% uninucleated and 20% binucleated microspores yielded the highest frequency of successful microspore cultures. Co-cultures with wheat, line ‘CY-45’, ovaries exhibited enhanced frequency of embryoid production than those with pepper ovaries. Differences in efficiency of isolated pepper microspore culture establishment were observed among different pepper genotypes. Green plantlets were regenerated from microspore-derived embryoids, but some were exhibited abnormal growth habits, such as leaf rosetting. A total of seven fertile microspore-derived plants were obtained, including three ‘Jariza’, three ‘Jaranda’, and a single ‘Szegedi 80’ plant.

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Abbreviations

FSOC:

Foreign species ovary co-culture

FDA:

Fluorescein diacetate

DH:

Doubled-haploid

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Acknowledgments

This work was supported by the Agency for Research Fund Management and Research Exploitation (KPI) and the National Office for Research and Technology (NKTH), project number DA_TECH_05-FPFTT005. The authors thank Erzsébet Juhász Fehér, Éva Kótai, Petra Majer, Mária Olasz, Zsuzsanna Kun and Zsuzsanna Ábrahám Táborosi for their conscientious work and Maria Isabel Garcia Pomar, Council of Extremadura Region Agricultural Research Service, “La Orden” Experimental Station, Badajoz, Spain, for the Spanish pepper genotypes.

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Correspondence to János Pauk.

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Lantos, C., Juhász, A.G., Somogyi, G. et al. Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat. Plant Cell Tiss Organ Cult 97, 285–293 (2009). https://doi.org/10.1007/s11240-009-9527-9

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Keywords

  • Capsicum annuum L.
  • Embryogenesis
  • Microspore culture
  • Ovary co-culture
  • Pepper