Plant Cell, Tissue and Organ Culture

, Volume 96, Issue 1, pp 11–18

Regeneration of different cultivars of common bean (Phaseolus vulgaris L.) via indirect organogenesis

  • Jesús Arellano
  • Sara Isabel Fuentes
  • Patricia Castillo-España
  • Georgina Hernández
Original Paper

DOI: 10.1007/s11240-008-9454-1

Cite this article as:
Arellano, J., Fuentes, S.I., Castillo-España, P. et al. Plant Cell Tiss Organ Cult (2009) 96: 11. doi:10.1007/s11240-008-9454-1

Abstract

A protocol for in vitro regeneration via indirect organogenesis for Phaseolus vulgaris cv. Negro Jamapa was established. The explants used were apical meristems and cotyledonary nodes dissected from the embryonic axes of germinating seeds. Several auxin/cytokinin combinations were tested for callus induction. The best callus production was obtained with medium containing 1.5 μM 2,4-dichlorophenoxyacetic acid. After 2 weeks of growth calli were transferred to shooting medium containing 22.2 μM 6-benzylaminopurine. Shoots regenerated with a frequency of approximately 0.5 shoots per callus, and upon transfer to rooting medium these shoots produced roots with 100% efficiency. Histological analyses of the regeneration process confirmed the indirect organogenesis pattern. Greenhouse grown regenerated plants showed normal development and were fertile. The protocol was reproducible for other nine P. vulgaris cultivars tested, suggesting a genotype independent procedure.

Keywords

Phaseolus vulgarisCommon bean Indirect organogenesis Plant regeneration 

Abbreviations

AM

Apical meristem

BAP

6-Benzylaminopurine

CIM

Callus induction medium

CN

Cotyledonary node

2,4-D

2,4-Dichlorophenoxyacetic acid

IAA

Indole-3-acetic acid

MES

2-(N-Morpholino) ethanesulfonic acid

MS

Murashige and Skoog medium

NAA

α-naphthaleneacetic acid

PVP-360

Polyvinylpyrrolidone 360,000 molecular weight

SM

Shooting medium

RM

Rooting medium

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Jesús Arellano
    • 1
  • Sara Isabel Fuentes
    • 1
  • Patricia Castillo-España
    • 2
  • Georgina Hernández
    • 1
  1. 1.Centro de Ciencias GenómicasUniversidad Nacional Autónoma de MéxicoMorelosMéxico
  2. 2.Centro de Investigación en BiotecnologíaUniversidad Autónoma del Estado de MorelosMorelosMéxico

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