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Plant Cell, Tissue and Organ Culture

, Volume 95, Issue 3, pp 373–379 | Cite as

The role of topolins in micropropagation and somaclonal variation of banana cultivars ‘Williams’ and ‘Grand Naine’ (Musa spp. AAA)

  • Michael W. Bairu
  • Wendy A. Stirk
  • Karel Doležal
  • Johannes van StadenEmail author
Original Paper

Abstract

The effect of the cytokinins mT (meta-topolin), mTR (meta-topolin riboside), MemT (meta-methoxy topolin) and MemTR (meta-methoxy topolin riboside) on micropropagation of banana cultivars ‘Williams’ and ‘Grand Naine’ was studied and compared to BA (6-benzylaminopurine). In vitro cultures, at the third sub-culture level, were purchased from African Biotechnologies (Pty) Ltd., South Africa. These were then sub-cultured on MS media containing 7.5, 15 and 30 μM of all the cytokinins tested. Results recorded after 6 weeks of growth demonstrated that there were statistically significant differences between the parameters analyzed for the treatments. Superior multiplication rates were recorded for mT and mTR treatments. This result was consistent when compared to BA at 22.2 μM (previously published standard concentration). Contrary to previous findings with other species, these cytokinins inhibited rooting. The effect on somaclonal variation was not significantly different when BA, mT and mTR were tested at the seventh multiplication cycle for ‘Williams’ banana. These results support the possible use of topolins as an alternative to BA for Cavendish banana tissue culture. The role of these cytokinins on somaclonal variation however, requires a more stringent investigation as the results obtained in this investigation could have been influenced by carry-over effects from the initial cultures.

Keywords

Abnormality index Banana Micropropagation Multiplication rate RAPD analysis Somaclonal variation Topolins 

Notes

Acknowledgments

We acknowledge the financial support of the National Research Foundation—South Africa, Grant Agency of the Czech Republic (GA522/06/0108) and Czech Ministry of Education (MSM 6198959216). We thank Marek Zatlaukal for help with the organic synthesis of the topolins.

References

  1. Bairu MW, Fennell CW, Van Staden J (2006) The effect of plant growth regulators on somaclonal variation in Cavendish banana (Musa AAA cv. ‘Zelig’). Sci Hortic (Amsterdam) 108:347–351. doi: 10.1016/j.scienta.2006.01.039 CrossRefGoogle Scholar
  2. Bairu MW, Stirk WA, Doležal K, Van Staden J (2007) Optimizing the micropropagation protocol for the endangered Aloe polyphylla: can meta-topolin and its derivatives serve as replacement for benzyladenine and zeatin? Plant Cell Tissue Organ Cult 90:15–23. doi: 10.1007/s11240-007-9233-4 CrossRefGoogle Scholar
  3. Baroja-Fernandez E, Aguirreolea J, Martínková H, Hanus J, Strnad M (2002) Aromatic cytokinins in micropropagated potato plants. Plant Physiol Biochem 40:217–224. doi: 10.1016/S0981-9428(02)01362-1 CrossRefGoogle Scholar
  4. Bogaert I, van Cauter S, Werbrouck SPO, Doležal K (2006) New aromatic cytokinins can make the difference. Acta Hortic 725:265–270Google Scholar
  5. Braun AC (1959) A demonstration of the recovery of the crown-gall tumour cell with the use of complex tumours of single-cell origin. Proc Natl Acad Sci USA 45:932–938. doi: 10.1073/pnas.45.7.932 PubMedCrossRefGoogle Scholar
  6. Cronauer SS, Krikorian AD (1984) Rapid multiplication of bananas and plantains by in vitro shoot tip culture. HortScience 19:234–235Google Scholar
  7. Damasco OP, Graham GC, Henry RJ, Adkins SW, Smith MK, Godwin ID (1996) RAPD detection of dwarf off-types in micropropagated ‘Cavendish’. (Musa spp. AAA) bananas. Plant Cell Rep 16:118–123. doi: 10.1007/BF01275464 CrossRefGoogle Scholar
  8. Damasco OP, Smith MK, Adkins SW (1998) Identification and characterization of dwarf off-types from micropropagated ‘Cavendish’ bananas. Acta Hortic 490:79–84Google Scholar
  9. Dore Swamy R, Srinivasa Rao NK, Chacko EK (1983) Tissue culture propagation of banana. Sci Hortic (Amsterdam) 18:247–252. doi: 10.1016/0304-4238(83)90028-6 CrossRefGoogle Scholar
  10. Escalona M, Cejas I, Gonzalez-Olmedo J, Capote I, Roels S, Canal MJ, Rodreguez R, Sandoval J, Debergh P (2003) The effect of meta-topolin on plantain propagation using a temporary immersion bioreactor. Infomusa 12:28–30Google Scholar
  11. Hills PN, Van Staden J (2002) An improved DNA extraction procedure for plant tissues with a high phenolic content. S Afr J Bot 68:549–550Google Scholar
  12. Jarret RL, Rodriguez W, Fernandez R (1985) Evaluation, tissue culture propagation and dissemination of ‘saba’ and ‘pelpita’ plantains in Costa-Rica. Sci Hortic (Amsterdam) 25:137–147. doi: 10.1016/0304-4238(85)90085-8 CrossRefGoogle Scholar
  13. Karp A (1994) Origin, causes and uses of variation in plant tissue culture. In: Vasil I, Thorpe T (eds) Plant cell and tissue culture. Kluwer Academic Publishers, Dordrecht, pp 139–151Google Scholar
  14. Krikorian AD, Cronauer SS (1984) Bananas. In: Sharp WR, Evance DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture crop species, vol 2. Macmillan Publishing Company, New York, pp 327–348Google Scholar
  15. Murashige T, Skoog F (1962) A revised medium for a rapid growth and bio-assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  16. Ramage CM, Borda AM, Hamill SD, Smith MK (2004) A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish banana (Musa Spp. AAA). Sci Hortic (Amsterdam) 103:145–151. doi: 10.1016/j.scienta.2004.04.015 CrossRefGoogle Scholar
  17. Reuveni O, Israeli Y (1990) Measures to reduce somaclonal variation in in vitro propagated bananas. Acta Hortic 275:307–313Google Scholar
  18. Richards EJ (1997) Preparation of plant DNA using CTAB. In: Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (eds) Short protocols in molecular biology, vol 3. Wiley, New York, pp 2.10–2.11Google Scholar
  19. Smith MK, Drew RA (1990) Growth and yield characteristics of dwarf off-types recovered from tissue cultured bananas. Aust J Exp Agric 30:575–578. doi: 10.1071/EA9900575 CrossRefGoogle Scholar
  20. Stover RH (1987) Somaclonal variation in Grand Naine and Saba bananas in the nursery and in the field. In: Perseley GJ, De Langhe EA (eds) Bananas and plantains breeding strategies. ACIAR proceeding no 21. ACIAR, Canberra, pp 136–139Google Scholar
  21. Vuylsteke DR (1998) Shoot-tip culture for the propagation, conservation, and distribution of Musa Germplasm. International Institute of Tropical Agriculture, Ibadan, Nigeria, 82 ppGoogle Scholar
  22. Vuylsteke DR, De Langhe E (1985) Feasibility of in vitro propagation of bananas and plantains. Trop Agric Trinidad 62:323–328Google Scholar
  23. Werbrouck SPO, van der Jeugt B, Dewitte W, Prinsen E, van Onckelen HA, Debergh PC (1995) The metabolism of benzyladenine in S. floribundum Schott ‘petite’ in relation to acclimatization problems. Plant Cell Rep 14:662–665. doi: 10.1007/BF00232734 CrossRefGoogle Scholar
  24. Werbrouck SPO, Strnad M, van Onckelen HA, Debergh PC (1996) Meta-topolin, an alternative to benzyladenine in tissue culture. Physiol Plant 98:291–297. doi: 10.1034/j.1399-3054.1996.980210.x CrossRefGoogle Scholar
  25. Wong WC (1986) In vitro propagation of banana (Musa spp.): initiation, proliferation and development of shoot tip cultures on defined media. Plant Cell Tissue Organ Cult 6:159–166. doi: 10.1007/BF00180799 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Michael W. Bairu
    • 1
  • Wendy A. Stirk
    • 1
  • Karel Doležal
    • 2
  • Johannes van Staden
    • 1
    Email author
  1. 1.Research Centre for Plant Growth and Development, School of Biological and Conservation SciencesUniversity of KwaZulu-Natal PietermaritzburgScottsvilleSouth Africa
  2. 2.Laboratory of Growth RegulatorsPalackỳ University and Institute of Experimental Botany AS CROlomouc-HoliceCzech Republic

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