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Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.)

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Abstract

Callus tissues originating from buds of mature Scots pine (Pinus sylvestris L.) trees exhibit the typical problem of browning, which leads to degeneration and death of the tissues. The effects of medium, origin (tree and location) and endophyte infection were studied on the browning and growth of bud-derived tissue cultures. The calli growing on medium with higher kinetin content and source of organic nitrogen, and originating from the southern location grew better and exhibited less browning. Endophytic microbial cells were detected in the brown callus tissues by transmission electron microscopy. The natural endophyte infection frequency of Scots pine buds was studied and found dependent on the tree, but not on the location. A well-growing, green callus line was artificially infected by an endophytic strain of Methylobacterium extorquens, and browning was not observed on solid media compared to the uninfected control clones of the same callus. However, suspension cultures started from the infected callus died faster than cultures started from the uninfected callus. The endophyte species composition and plant genotype together with tissue culture conditions are the key factors for gaining plant tissue cultures with high regeneration capacity.

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Acknowledgments

This work was supported by Niemi Foundation and Academy of Finland (No. 105586, 118569).

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Authors and Affiliations

  1. Department of Biology, University of Oulu, P·O. Box 3000, 90014, Oulu, Finland

    Anna Maria Pirttilä, Janne J. Koskimäki, Esa Hohtola & Anja Hohtola

  2. Institute of Agroecology of UAAS, Metrologichna st., 12, Kyiv, 03143, Ukraine

    Olga Podolich

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  1. Anna Maria Pirttilä
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  2. Olga Podolich
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Correspondence to Anna Maria Pirttilä.

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Pirttilä, A.M., Podolich, O., Koskimäki, J.J. et al. Role of origin and endophyte infection in browning of bud-derived tissue cultures of Scots pine (Pinus sylvestris L.). Plant Cell Tiss Organ Cult 95, 47–55 (2008). https://doi.org/10.1007/s11240-008-9413-x

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  • DOI: https://doi.org/10.1007/s11240-008-9413-x

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