An expressed sequence tag (EST) analysis approach was undertaken to identify the genes involved in photosynthesis and metabolism of Bambusa edulis Murno, an important bamboo crop species in Asia. The cDNA library was constructed from in vitro-grown multiple shoots. Sequencing of the cDNA clones generated 987 5′-end high-quality expressed sequence tags (ESTs, Accession number: FG551848–FG552834), of which 792 were revealed by sequence comparison to be unique. A BLASTX analysis showed that 645 of these genes were similar to genes present in the NCBI database. A total of 389 genes were most similar to rice genes. Nine genes had an E-value of zero. Functional classification of these genes by the Munich Information Center for Protein Sequences (MIPS) showed that most of the genes were related to metabolism and subcellular localization processes. PSI assembly protein (Beycf3) expression was absent in the albino mutant, while PSII 43-kDa protein (BepsbC), chlorophyll synthase (BeCAO) and PSII 10-kDa protein (BePsbR) expression were reduced. These data suggest that in this chloroplast genome-aberrated mutant, both chloroplast-encoded and nucleus-encoded genes were repressed and that this library will be useful for further investigation of bamboo.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
- EST :
Expression Sequence Tag
- MIPS :
Munich Information Center for Protein Sequences
- NCBI :
National Center for Biotechnology
- TDZ :
Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W et al (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. doi:10.1093/nar/25.17.3389
Collett H, Butowt R, Smith J, Farrant J, Illing N (2003) Photosynthetic genes are differentially transcribed during the dehydration-rehydration cycle in the resurrection plant, Xerophyta humilis. J Exp Bot 54:2593–2595. doi:10.1093/jxb/erg285
Dunford R, Walden RM (1991) Plastid genome structure and plastid-related transcript levels in albino barley plants derived from another culture. Curr Genet 20:339–347. doi:10.1007/BF00318524
Espineda CE, Linford AS, Devine D, Brusslan JA (1999) The AtCAO gene, encoding chlorophyll a oxygenase, is required for chlorophyll b synthesis in Arabidopsis thaliana. Proc Natl Acad Sci USA 96:10507–10511. doi:10.1073/pnas.96.18.10507
Hsieh MH, Goodman HM (2005) The Arabidopsis IspH homolog is involved in the plastid nonmevalonate pathway of isoprenoid biosynthesis. Plant Physiol 138:641–653. doi:10.1104/pp. 104.058735
Lin CS, Chang WC (1998) Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of regenerated plantlets. Plant Cell Rep 17:617–620. doi:10.1007/s002990050453
Lin CS, Lai YH, Sun CW, Liu NT, Tsay HS, Chang WC et al (2006) Identification of ESTs differentially expressed in green and albino mutant bamboo (Bambusa edulis) by suppressive subtractive hybridization (SSH) and microarray analysis. Plant Cell Tissue Organ Cult 86:169–175. doi:10.1007/s11240-006-9105-3
Lin CS, Liu NT, Liao DC, Yu JS, Tsao CH, Lin CH et al (2008) Differential protein expression of two photosystem II subunits, PsbO and PsbP in an albino mutant of Bambusa edulis Murno with chloroplast DNA aberration. J Am Soc Hortic Sci 133:270–277
Liu NT, Wu FH, Tsay HS, Chang WC, Lin CS (2007) Chloroplast genome aberration in micropropagation-derived albino mutants, ab1 and ab2. Plant Cell Tissue Organ Cult 88:147–156. doi:10.1007/s11240-006-9182-3
Martin W, Stoebe B, Goremykin V, Hansmann S, Hasegawa M, Kowallik K (1998) Gene transfer to the nucleus and the evolution of chloroplasts. Nature 393:162–165. doi:10.1038/30234
Mewes HW, Heumann K, Kaps A, Mayer K, Pfieffer F, Stocker S et al (1999) MIPS: a databse for genomes and protein sequences. Nucleic Acids Res 27:44–48. doi:10.1093/nar/27.1.44
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
Naver H, Boudreaul E, Rochaix J-D (2001) Functional studies of ycf3 its role in assembly of photosystem I and interactions with some of its subunits. Plant Cell 13:2731–2745
Oono Y, Seki M, Nanjo T, Narusaka M, Fujita M, Satoh R et al (2003) Monitoring expression profiles of Arabidopsis gene expression during rehydration process after dehydration using c. 7000 full-length cDNA microarray. Plant J 34:868–887. doi:10.1046/j.1365-313X.2003.01774.x
Teng YS, Su YS, Chen LJ, Lee YJ, Hwang I, Li HM (2006) Tic21 is an essential translocon component for protein translocation across the chloroplast inner envelope membrane. Plant Cell 18:2247–2257. doi:10.1105/tpc.106.044305
Verica JA, Maximova SN, Strem MD, Carlson JE, Bailey BA, Guiltinan MJ (2004) Isolation of ESTs from cacao (Theobroma cacao L.) leaves treated with inducers of the defense response. Plant Cell Rep 23:404–413. doi:10.1007/s00299-004-0852-5
Vranova E, Atichartpongkul S, Villarroel R, Van Montagu M, Inze D, Van Camp W (2002) Comprehensive analysis of gene expression in Nicotiana tabacum leaves acclimated to oxidative stress. Proc Natl Acad Sci USA 99:10870–10875. doi:10.1073/pnas.152337999
About this article
Cite this article
Liu, N., Wu, F., Tsay, H. et al. Establishment of a cDNA library from Bambusa edulis Murno in vitro-grown shoots. Plant Cell Tiss Organ Cult 95, 21–27 (2008). https://doi.org/10.1007/s11240-008-9409-6
- cDNA library
- Albino mutant