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Plant Cell, Tissue and Organ Culture

, Volume 94, Issue 3, pp 305–311 | Cite as

Toxic effect of nitroesters on plant tissue cultures

  • Radka Podlipná
  • Zuzana Fialová
  • Tomáš VaněkEmail author
Original Paper

Abstract

Nitroglycerine (NG) is used in manufacture of dynamite, gunpowder, and rocket propellants, and as a therapeutic agent primarily to angina pectoris. Ethylene glycol dinitrate (EGDN) is an explosive ingredient (60–80%) in dynamite along with nitroglycerine (40–20%). Both compounds are pollutants, and due to recalcitrance to biodegradation they persist in the environment. In our study we concentrated on the phytotoxicity of these compounds, looking in particular at their effects on germination, growth and development. We used mustard (Sinapis alba) seedlings, in vitro cultivated regenerants of Juncus inflexus and Phragmites australis and suspension cultures of flax (Linum usitatissimum). The flax cells absorbed NG and transformed it to both dinitroglycerine isomers. By measuring their effect on the length of primary root of white mustard seedlings we demonstrated that the toxicity of these degradation products was lower than the original compound. The phytotoxic effect of EGDN on wetland plant species is described for the first time. High concentrations of EGDN (500 mg l−1 and higher) in the medium induced wilting and browning and subsequent death of reed and rush plants.

Keywords

Nitroglycerine Ethylene glycol dinitrate Phytotoxicity Phytoremediation 

Abbreviations

2, 4-D

Dichlorophenoxyacetic acid

DNG

Dinitroglycerine

EGDN

Ethylene glycol dinitrate

MNG

Mononitroglycerine

NG

Nitroglycerine (trinitroglycerine)

TNT

Trinitrotoluene

TTC

2, 3, 5-Triphenyltetrazolium chloride

Notes

Acknowledgements

This work was supported by projects MPO FT-TA3/118 and MYES 1MO6030

References

  1. Adams N, Carroll D, Madalinski K, Rock S, Wilson T, Pivetz B (2000) Introduction to phytoremediation. Report EPA/600/R-99/107. U.S.. Environmental Protection Agency, National Risk Management Research Laboratory, Cincinnati, OHGoogle Scholar
  2. Christodoulatos C, Bhaumik S, Brodman BW (1997) Anaerobic biodegradation of nitroglycerin. Water Res 31(6):1462–1470. doi: 10.1016/S0043-1354(96)00390-9 CrossRefGoogle Scholar
  3. Čihalík J, Čulíková H, Dusílek P, Finfrlová P, Konrád V, Podlešáková E et al (1989) Metodický návod pro hodnocení rizikovosti tuhého průmyslového odpadu. Acta Hygien. Epidemiol Microbiol 55:38–41Google Scholar
  4. Cunningham SD, Anderson TA, Schwab AP, Hsu FC (1996) Phytoremediation of soils contaminated with organic pollutants. Adv Agron 56:55–114. doi: 10.1016/S0065-2113(08)60179-0 CrossRefGoogle Scholar
  5. Ellis HV, Hong CB, Lee CC, Dacre JC, Glennon JP (1984) Subacute and chronic toxicity studies of trinitroglycerin in dogs, rats, and mice. Fundam Appl Toxicol 4:248–260. doi: 10.1016/0272-0590(84)90126-X PubMedCrossRefGoogle Scholar
  6. French CE, Rosser SJ, Davies GJ, Nicklin S, Bruce NC (1999) Biodegradation of explosives by transgenic plants expressing pentaerythritol tetranitrate reductase. Nat Biotechnol 17:491–494. doi: 10.1038/8673 PubMedCrossRefGoogle Scholar
  7. Fukuchi Y (1981) Nitroglycol concentrations in blood and urine of workers engaged in dynamite production. Int Arch Occup Environ Health 48:339–346. doi: 10.1007/BF00378682 PubMedCrossRefGoogle Scholar
  8. Goel A, Kumar G, Payne GF, Dube SK (1997) Plant cell biodegradation of a xenobiotic nitrate ester, nitroglycerin. Nat Biotechnol 15:174–177. doi: 10.1038/nbt0297-174 PubMedCrossRefGoogle Scholar
  9. Hannink NK, Rosser SJ, Bruce NC (2002) Phytoremediation of explosives. Crit Rev Plant Sci 21(5):511–538. doi: 10.1080/0735-260291044340 CrossRefGoogle Scholar
  10. Imoto S, Kuramoto M, Iwabuchi K, Nagai H, Shimpo K (1986) Percutaneous chronic toxicity study of 10% nitroglycerin (NT-1 ointment) in rabbits. J Toxicol Sci 11(2):31–57PubMedGoogle Scholar
  11. Johansson P, Ehrenstrom F, Ungell AL (1987) Study on Adrenergic Function after Development of Tolerance to Ethylene Glycol Dinitrate (EGDN) in Rats. Pharmacol Toxicol 61(3):172–181PubMedGoogle Scholar
  12. Kanerva L, Laine R, Jolanki R, Tarvainen K, Estlander T, Helander I (1991) Occupational allergic contact dermatitis caused by nitroglycerin. Contact Derm 24:356–362. doi: 10.1111/j.1600-0536.1991.tb01750.x PubMedCrossRefGoogle Scholar
  13. Lamm SH, Grumski KS, Fethers RF (1993) Short-term exposures to ethylene glycol dinitrate concentrations greater than 0.4 mg/m3 show no evidence of nitrate-induced headaches. Am J Epidemiol 138:653Google Scholar
  14. Lowry BP, Bradfield JF, Carroll RG, Brewer K, Meggs WJ (2001) A controlled trial of topical nitroglycerin in a New Zealand white rabbit model of brown recluse spider envenomation. Ann Emerg Med 37(2):161–165. doi: 10.1067/mem.2001.113031 PubMedCrossRefGoogle Scholar
  15. Marshall SJ, White GF (2001) Complete denitration of nitroglycerin by bacteria isolated from a washwater soakaway. Appl Environ Microbiol 67(6):2622–2626. doi: 10.1128/AEM.67.6.2622-2626.2001 PubMedCrossRefGoogle Scholar
  16. Miller WE, Peterson SA, Greene JC, Callahan CA (1985) Comparative toxicology of laboratory organisms for assessing hazardous waste sites. J Environ Qual 14:569–574CrossRefGoogle Scholar
  17. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497. doi: 10.1111/j.1399-3054.1962.tb08052.x CrossRefGoogle Scholar
  18. Pivetz BE (2001) Ground water issue: phytoremediation of contaminated soil and ground water at hazardous waste sites. EPA/540/S-01/500, pp 1–36. http://oaspub.epa.gov/eims/direntrpt.report?p_deid=23491&p_chk=65104
  19. Sangwan RS, Gorenflot R (1975) In vitro culture of Phragmites tissue. Callus formation, organ differentation and cell suspension culture. Z Pflanzenphysiol 75:256–269Google Scholar
  20. Santoro A, Rovati LC, Setnikar I (2001) Assessment of skin safety of a new glyceryl trinitrate transdermal patch. Arzneimittelforschung 51:29–37PubMedGoogle Scholar
  21. Schnoor JL, Licht LA, McCutcheon SC, Wolfe NL, Carreira LH (1995) Phytoremediation of organic and nutrient contaminants. Environ Sci Technol 29:318A–323A. doi: doi:10.1021/es00007a002 CrossRefGoogle Scholar
  22. Stayner LT, Dannenberg AL, Thun M, Reeve G, Bloom TF, Boeniger M et al (1992) Cardiovascular mortality among munitions workers exposed to nitroglycerin and dinitrotoluene. Scand J Work Environ Health 18:34–43PubMedGoogle Scholar
  23. Tai T, Tsuruta H (1997) The effects of nitroglycol on rat isolated cardiac muscle. Ind Health 35:515–518. doi: 10.2486/indhealth.35.515 PubMedCrossRefGoogle Scholar
  24. Vaněk T, Nepovím A, Podlipná R, Zeman S, Vágner M (2003) Phytoremediation of selected explosives. Water Air Soil Pollut 3:259–267Google Scholar
  25. Ye J, Singh A, Ward OP (2004) Biodegradation of nitroaromatics and other nitrogen-containing xenobiotics. World J Microbiol Biotechnol 20:117–135. doi: 10.1023/B:WIBI.0000021720.03712.12 CrossRefGoogle Scholar
  26. Zalta AH, Karlin KM, Zimmerman TJ (1985) The effect of nitroglycerin ointment on the external ocular structures of rabbits. J Ocul Pharmacol 1(1):71–77PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Radka Podlipná
    • 1
  • Zuzana Fialová
    • 1
  • Tomáš Vaněk
    • 1
    Email author
  1. 1.Laboratory of Plant Biotechnologies, Joint Laboratory of Institute of Experimental Botany AS CR, v.v.i. and Research Institute of Crop Production, v.v.i.Prague 6Czech Republic

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