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Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea

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Abstract

Procedures for asymbiotic seed germination and seedling acclimatization were developed for Bletia purpurea, a threatened North America native terrestrial orchid. Six asymbiotic orchid seed germination media (Knudson C, PhytoTechnology Orchid Seed Sowing Medium, Malmgren Modified Terrestrial Orchid Medium, Vacin & Went Modified Orchid Medium, ½-strengh Murashige & Skoog, and BM-1 Terrestrial Orchid Medium) were examined for their effectiveness in promoting seed germination and protocorm development of B. purpurea in either a 0/24 h or 16/8 h L/D photoperiod. Germination occurred regardless of medium or photoperiod treatment. However, advanced seedling development (Stage 6) only occurred on Vacin & Went Modified Orchid Medium in the 16/8 h L/D photoperiod. Further effects of photoperiod on in vitro seedling development were also examined. Shoot length, leaf width, root number and length, and fresh weight and dry weight in the 16/8 h L/D photoperiod were all significantly different when compared to the 8/16 h and 12/12 L/D photoperiods. In vitro seedlings were readily acclimatized to greenhouse conditions. Seedlings showed high survival all potting media. Seedlings acclimatized in Fafard Mix 4 potting medium developed significantly longer roots. Corm formation occurred regardless of potting media used.

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Abbreviations

½MS:

1/2-Strength Murashige and Skoog

BM-1:

Terrestrial Orchid Medium

dd:

Distilled deionized

FPNWR:

Florida Panther National Wildlife Refuge

KC:

Knudson C

L/D:

Light/dark

MM:

Malmgren Modified Terrestrial Orchid Medium

P723:

PhytoTechnology Orchid Seed Sowing Medium

VW:

Vacin & Went Modified Orchid Medium

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Acknowledgements

The authors thank the Florida Panther National Wildlife Refuge—US Fish and Wildlife Service for providing financial and logistical support for this project. We also thank the two anonymous reviewers for their helpful comments. Brand names are provided for reference only; the authors do not solely endorse these particular products.

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Correspondence to Daniela Dutra.

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Dutra, D., Johnson, T.R., Kauth, P.J. et al. Asymbiotic seed germination, in vitro seedling development, and greenhouse acclimatization of the threatened terrestrial orchid Bletia purpurea . Plant Cell Tiss Organ Cult 94, 11–21 (2008). https://doi.org/10.1007/s11240-008-9382-0

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Keywords

  • Orchid
  • Seed germination
  • Native
  • Conservation
  • Pine pink