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Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene

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Abstract

Laccases are copper-containing glycoproteins, which are widespread in higher plants as multigene families. To gain more insight in the function of laccases in plants, especially potential role in lignification, we produced transgenic poplar plants overexpressing a cotton laccase cDNA (GaLAC1) under the control of the cauliflower mosaic virus 35S promoter. As compared with untransformed control plants, transgenic plants exhibited a 2.1- to 13.2-fold increased laccase activity, whereas plant growth rate and morphological characters remained similar to control plants. A 2.1–19.6% increase in total lignin content of the stem was found in transgenic plants. Moreover, transgenic plants showed a dramatically accelerated oxidation rate of phenolics, without obvious change in total phenolic content. Our data suggested that GaLAC1 may participate in lignin synthesis and phenolic metabolism in plants. The present work provided a new genetic evidence for the involvement of plant laccases in lignification.

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Abbreviations

ABTS:

2,2-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)

AS:

Acetosyringone

BAP:

Benzylaminopurine

CaMV 35S promoter:

Cauliflower mosaic virus 35S promoter

IAA:

Indole-3 acetic acid

PSK-α:

Phytosulfokine-α

ZT:

Zeatin

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Acknowledgments

We sincerely thank to Dr. Xiaoya Chen for providing GaLAC1 gene, Dr. Bin Luo for his help with the experiments, Dr. Yoshikatsu Matsubayashi for providing PSK-α, Dr. Jeffrey Dean for providing his electronic version article, Dr. Zhangqun Ye for providing antibiotic and Dr. Longcheng Li for critically reading of the manuscript.

Author information

Correspondence to Zhiming Wei.

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Wang, J., Wang, C., Zhu, M. et al. Generation and characterization of transgenic poplar plants overexpressing a cotton laccase gene. Plant Cell Tiss Organ Cult 93, 303 (2008). https://doi.org/10.1007/s11240-008-9377-x

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Keywords

  • Laccase
  • Lignin
  • Phenolic metabolism
  • Populus
  • Transgenic poplars