Paenibacillus—a predominant endophytic bacterium colonising tissue cultures of woody plants
- 598 Downloads
High densities of endophytic bacteria were found in plant material from poplar, larch and spruce that had been micropropagated for at least 5 years. The majority of these bacteria were assigned to the genus Paenibacillus based on the sequencing of the 16S rRNA genes. Other endophytic bacteria such as Methylobacterium, Stenotrophomonas or Bacillus could also be found but only in some tissue cultures. Certain species or strains of Paenibacillus, especially those with a close relationship to P. humicus, seemed to accumulate under in vitro conditions without visible negative influences on the plant’s development. Poplar microcuttings inoculated with the endophytic Paenibacillus isolate 22 showed significantly more roots per cutting and higher root length in comparison to the control plants after 3 weeks.
KeywordsMicropropagation Poplar Root growth promotion
Colony forming units
This work was supported by grant 0313285I from the Federal Ministry of Education and Research. We are grateful to Mrs. H. Enkisch for her excellent technical assistance. We also thank Prof. Yang from the Agricultural University Hebei, Baoding for providing the poplar clone 741 including several transgenic lines and the white poplar clone, and Dr. M. Fladung and Dr. I. Zaspel for providing plant materials from poplar and black locust clones, respectively.
- Ewald D (2007) Micropropagation of Larix species via organogenesis. In: Jain SM, Häggman H (eds) Protocols for micropropagation of woody trees and fruits. Springer, DordrechtGoogle Scholar
- Ewald D, Hu JJ (2003) Efficiency in the formation of stable embryogenic lines and somatic embryo regeneration in Norway spruce and hybrid larch. Sci Silvae Sin 39:53–62Google Scholar
- Ewald D, Hu JJ (2007) Influence of cytokinin and ammonium nitrate on elongation of adventitious buds in Norway Spruce (Picea abies). Sci Silvae Sin 43:28–43Google Scholar
- Ewald D, Süss R (1993) A system for repeatable formation of elongating adventitious buds in Norway spruce tissue cultures. Silvae Genet 42:169–175Google Scholar
- Ewald D, Zaspel I, Naujoks G et al (2000) Endogenous bacteria in tissue cultures of conifers – appearance and action. In: Cassells AC, Doyle BM, Curry RF (eds) Proceedings of the International Symposium on Methods and Markers for Quality Assurance in Micro propagation. Acta Hort 530:137–145Google Scholar
- Leifert C, Waites WM, Nicholas JR (1989) Bacterial contaminants of micropropagated plant cultures. J Appl Bacteriol 67:353–361Google Scholar
- Naujoks G, Zaspel I, Behrendt U (2000) Microorganisms acting in tissue culture of black locust (Robinia pseudoacacia L.). In: Cassells AC, Doyle BM, Curry RF (eds) Proceedings of the International Symposium on Methods and Markers for Quality Assurance in Micro propagation. Acta Hort 530:129–135Google Scholar
- Schneck V, Ewald D (2001) Growth and performance of micropropagated hybrid larch clones. Silvae Genet 50:240–243Google Scholar
- Tian YC, Zheng JB, Yu HM et al (2000) Studies of transgenic hybrid poplar carrying two insect-resistant genes. Acta Bot Sin 42:263–268Google Scholar