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Plant Cell, Tissue and Organ Culture

, Volume 93, Issue 3, pp 347–351 | Cite as

Paenibacillus—a predominant endophytic bacterium colonising tissue cultures of woody plants

  • Kristina Ulrich
  • Thomas Stauber
  • Dietrich Ewald
Research Note

Abstract

High densities of endophytic bacteria were found in plant material from poplar, larch and spruce that had been micropropagated for at least 5 years. The majority of these bacteria were assigned to the genus Paenibacillus based on the sequencing of the 16S rRNA genes. Other endophytic bacteria such as Methylobacterium, Stenotrophomonas or Bacillus could also be found but only in some tissue cultures. Certain species or strains of Paenibacillus, especially those with a close relationship to P. humicus, seemed to accumulate under in vitro conditions without visible negative influences on the plant’s development. Poplar microcuttings inoculated with the endophytic Paenibacillus isolate 22 showed significantly more roots per cutting and higher root length in comparison to the control plants after 3 weeks.

Keywords

Micropropagation Poplar Root growth promotion 

Abbreviations

Cfu

Colony forming units

Notes

Acknowledgements

This work was supported by grant 0313285I from the Federal Ministry of Education and Research. We are grateful to Mrs. H. Enkisch for her excellent technical assistance. We also thank Prof. Yang from the Agricultural University Hebei, Baoding for providing the poplar clone 741 including several transgenic lines and the white poplar clone, and Dr. M. Fladung and Dr. I. Zaspel for providing plant materials from poplar and black locust clones, respectively.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kristina Ulrich
    • 1
  • Thomas Stauber
    • 1
  • Dietrich Ewald
    • 1
  1. 1.Federal Research Centre for Forestry and Forest ProductsInstitute for Forest Genetics and Forest Tree BreedingWaldsieversdorfGermany

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