Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 92, Issue 1, pp 107–111 | Cite as

Cryopreservation of shoot-tips by droplet vitrification applicable to all taro (Colocasia esculenta var. esculenta) accessions

  • Rajnesh SantEmail author
  • Bart Panis
  • Mary Taylor
  • Anand Tyagi
Research Note

Abstract

The application of the droplet vitrification cryopreservation technique to taro accessions from a range of Asia Pacific countries is presented. The optimum protocol involves excision of about 0.8 mm shoot-tips from in vitro plants, 20–40 min PVS2 exposure at 0°C followed by rapid plunge into liquid nitrogen. Thawing was done at room temperature (25°C) and shoot-tips inoculated on MS medium with 0.1 M sucrose regenerated into plantlets 4–6 weeks later. This new droplet vitrification protocol improved the mean post-thaw regeneration rates to 73–100% from 21–30% obtained with the previous cryo-vial vitrification protocol.

Keywords

Taro Cryopreservation Shoot-tips Droplet vitrification 

Notes

Acknowledgments

Funding for this research was provided by AusAID through the Taro Genetic Conservation and Utilization (TaroGen) project. The training on droplet vitrification technique provided by Dr Panis was funded by INIBAP and AusAID.

References

  1. Leunufna S, Keller ERJ (2003) Investigating a new cryopreservation protocol for yams (Dioscorea spp.). Plant Cell Rep 21:1159–1166PubMedCrossRefGoogle Scholar
  2. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497CrossRefGoogle Scholar
  3. Panis B, Piette B, Swennen R (2005) Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Sci 168:45–55CrossRefGoogle Scholar
  4. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanaka) cooled to −196°C. J Plant Physiol 137:465–470Google Scholar
  5. Sant R, Taylor M, Tyagi A (2006) Cryopreservation of in vitro-grown shoot-tips of tropical taro (Colocasia esculenta var. esculenta) by vitrification. Cryo-Lett 27:133–142Google Scholar
  6. Schäfer-Menuhr A, Schumacher HM, Mix-Wagner G (1997) Cryopreservation of potato cultivars: design of a method for routine application in genebanks. Acta Hortic 447:477–482Google Scholar
  7. Takagi H, Thinh NT, Islam OM, Senboku T, Sakai A (1997) Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L) Schott) by vitrification.1. Investigation of basic conditions of the vitrification procedure. Plant Cell Rep 16:594–599Google Scholar
  8. Thinh NT (1997) Cryopreservation of germplasm of vegetatively propagated tropical monocots by vitrification. PhD Dissertation, Kobe University, JapanGoogle Scholar
  9. Touchell DH, Chiang VL, Tsai CJ (2002) Cryopreservation of embryogenic cultures of Picea mariana (black spruce) using vitrification. Plant Cell Rep 21:118–124CrossRefGoogle Scholar
  10. Towill LE, Bonnart R (2003) Cracking in a vitrification solution during cooling or warming does not affect growth of cryopreserved mint shoot tips. Cryo-Lett 24:341–346Google Scholar
  11. Tuia VS (1997) In vitro multiplication of taro (Colocasia esculenta var. esculanta L. Schott). MSc Dissertation, The University of the South Pacific, SamoaGoogle Scholar
  12. Vandenbussche B, Weyens G, De Proft M (2000) Cryopreservation of in vitro sugar beet (Beta vulgaris L.) shoot tips by a vitrification technique. Plant Cell Rep 19:1064–1068CrossRefGoogle Scholar
  13. Wang YL, Fan MJ, Liaw SI (2005) Cryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification. Bot Bull Acad Sinica 46:29–34Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Rajnesh Sant
    • 1
    Email author
  • Bart Panis
    • 2
  • Mary Taylor
    • 3
  • Anand Tyagi
    • 4
  1. 1.School of Life SciencesQueensland University of TechnologyBrisbaneAustralia
  2. 2.Laboratory of Tropical Crop Improvement, Division of Crop BiotechnicsK.U. LeuvenLeuvenBelgium
  3. 3.Secretariat of the Pacific CommunitySuvaFiji
  4. 4.Department of BiologyThe University of the South PacificSuvaFiji

Personalised recommendations