Plant Cell, Tissue and Organ Culture

, Volume 91, Issue 1, pp 9–19

Influence of galactoglucomannan oligosaccharides on root culture of Karwinskia humboldtiana

  • Karin Kollárová
  • Desana Lišková
  • Alexander Lux
Original Paper

DOI: 10.1007/s11240-007-9272-x

Cite this article as:
Kollárová, K., Lišková, D. & Lux, A. Plant Cell Tiss Organ Cult (2007) 91: 9. doi:10.1007/s11240-007-9272-x

Abstract

Galactoglucomannan oligosaccharides (GGMOs) activity in K. humboldtiana root culture has been determined. GGMOs inhibited adventitious root growth and lateral root induction in contrast to IAA, IBA, and NAA stimulating effect in these processes. Similarly, the combination of GGMOs with natural auxins (IAA, IBA) evoked an inhibition of adventitious root growth and lateral root induction that depended on the oligosaccharides concentration and the type of auxin. The growth stimulating effect of the synthetic auxin, NAA, in adventitious roots was negatively affected by GGMOs, but they were without influence on lateral root induction. The presence of oligosaccharides triggered lateral root position on adventitious roots and the anatomy of adventitious roots (diameter, proportion of primary cortex to the central cylinder, number and size of primary cortical cells, intercellular spaces, and the number of starch grains in cells of primary cortex) in dependence on their coactions with auxin.

Keywords

Anatomy IAA IBA Induction and growth of roots Karwinskia humboldtiana NAA 

Abbreviations

ANOVA

Analysis of variance

d.p.

Degree of polymerisation

2,4-D

2,4-Dichlorophenoxyacetic acid

GA3

Gibberellic acid

GGMOs

Galactoglucomannan oligosaccharides

IAA

Indole-3-acetic acid

IBA

Indole-3-butyric acid

LSD test

Least significant difference test

NAA

1-Naphthaleneacetic acid

SE

Standard error

WPM

Woody plant medium

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Karin Kollárová
    • 1
  • Desana Lišková
    • 1
  • Alexander Lux
    • 1
    • 2
  1. 1.Institute of ChemistrySlovak Academy of SciencesBratislavaSlovakia
  2. 2.Department of Plant Physiology, Faculty of Natural SciencesComenius University in BratislavaBratislavaSlovakia

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