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Plant regeneration from leaf protoplasts of Solanum virginianum L. (Solanaceae)

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Abstract

Leaves of Solanum virginianum plants were used for protoplast isolation. To support cell wall formation and cell division, protoplasts were cultured in thin alginate layers floated in liquid medium. When protoplasts were plated at a density of 1.0 × 106/ml in Kao and Michyaluk (KMp8) medium supplemented with 0.5 mg/l zeatin, 1.0 mg/l 2,4-dichlorophenoxyacetic acid, and 1.0 mg/l α-naphthaleneacetic acid, 42.3% of the dividing cells developed microcalli in 3–4 weeks. Shoot formation via organogenesis of protoplast-derived calli was achieved for 28% of calli transferred to solidified KMp8 medium supplemented with 2.0 g/l zeatin and 0.1 mg/l 3-indol acetic acid in about 2 weeks. Further shoot development was observed in Murashige and Skoog (MS) medium without growth regulators and roots were induced after transfer to MS medium containing 1.0 mg/l 3-indol butyric acid. Regenerated plants have normal morphology.

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Fig. 1

Abbreviations

MS:

Murashige and Skoog medium

KMp8:

Kao and Michyaluk medium

PVP:

Polyvinylpirrolidone

IAA:

3-indol acetic acid

NAA:

α-naphthaleneacetic acid

IBA:

3-indol butyric acid

2,4-d :

2,4-dichlorophenoxyacetic acid

Kin:

Kinetin

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Correspondence to A. Furini.

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Borgato, L., Pisani, F. & Furini, A. Plant regeneration from leaf protoplasts of Solanum virginianum L. (Solanaceae). Plant Cell Tiss Organ Cult 88, 247–252 (2007). https://doi.org/10.1007/s11240-006-9196-x

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Keywords

  • Organogenesis
  • Plant regeneration
  • Protoplast culture
  • Solanum virginianum