Plant Cell, Tissue and Organ Culture

, Volume 88, Issue 2, pp 157–165 | Cite as

Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia

  • Adam M. Dimech
  • Rob Cross
  • Rebecca Ford
  • Paul W. J. Taylor
Original Paper

Abstract

The physiological effects of three auxins [indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-d)] and two cytokinins [thidiazuron (TDZ) and N6-benzylaminopurine (NAA)] on in vitro morphogenesis of Doryanthes excelsa were measured. Longitudinal bud sections derived from immature inflorescences were used as a source of explants. Callus regeneration was observed at the highest frequencies (46.2%) when grown on media containing 50 μmol L-1 NAA and 0.5 μmol L−1 TDZ. Adventitious shoot organogenesis was observed at the highest frequency (56.8%) when grown on media containing 0.5 μmol L−1 NAA and 50 μmol L−1 TDZ. Regenerated shoots were rooted ex vitro after 6 weeks when dipped in a solution of 50 μmol L−1 NAA or no plant growth regulators were applied.

Keywords

Callus Shoot organogenesis Doryanthes excelsa Micropropagation Auxin Cytokinin Gymea lily 

Abbreviations

2,4-d

2,4-dichlorophenoxyacetic acid

BA

N6-benzylaminopurine

IBA

Indole−3-butyric acid

MES

n-morpholinoethanesulfonic acid

MS

Murashige and Skoog (1962) medium

NAA

α-naphthaleneacetic acid

TDZ

Thidiazuron [1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea]

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Adam M. Dimech
    • 1
    • 2
  • Rob Cross
    • 1
  • Rebecca Ford
    • 2
  • Paul W. J. Taylor
    • 2
  1. 1.Royal Botanic Gardens MelbourneSouth YarraAustralia
  2. 2.Faculty of Land and Food ResourcesUniversity of MelbourneRoyal ParadeAustralia

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