Plant Cell, Tissue and Organ Culture

, Volume 88, Issue 2, pp 157–165 | Cite as

Micropropagation of Gymea Lily (Doryanthes excelsa Corrêa) from New South Wales, Australia

  • Adam M. DimechEmail author
  • Rob Cross
  • Rebecca Ford
  • Paul W. J. Taylor
Original Paper


The physiological effects of three auxins [indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-d)] and two cytokinins [thidiazuron (TDZ) and N6-benzylaminopurine (NAA)] on in vitro morphogenesis of Doryanthes excelsa were measured. Longitudinal bud sections derived from immature inflorescences were used as a source of explants. Callus regeneration was observed at the highest frequencies (46.2%) when grown on media containing 50 μmol L-1 NAA and 0.5 μmol L−1 TDZ. Adventitious shoot organogenesis was observed at the highest frequency (56.8%) when grown on media containing 0.5 μmol L−1 NAA and 50 μmol L−1 TDZ. Regenerated shoots were rooted ex vitro after 6 weeks when dipped in a solution of 50 μmol L−1 NAA or no plant growth regulators were applied.


Callus Shoot organogenesis Doryanthes excelsa Micropropagation Auxin Cytokinin Gymea lily 



2,4-dichlorophenoxyacetic acid




Indole−3-butyric acid


n-morpholinoethanesulfonic acid


Murashige and Skoog (1962) medium


α-naphthaleneacetic acid


Thidiazuron [1-phenyl-3-(1,2,3-thiadiazol-5-yl) urea]



A. M. Dimech wishes to thank Mr. Greg Miles of Princeton Nurseries (Mount White, NSW, Australia) for his generous assistance. This research was jointly funded by the Rural Industries Research and Development Corporation and the Commonwealth Government of Australia through an Australian Postgraduate Award.


  1. Boltenkov EV, Rybin VG, Zarembo EV (2004) Specific features of cultivation of Iris ensata thunb. Callus tissue. Appl Microbiol Biotechnol 40(2):206–212CrossRefGoogle Scholar
  2. Boltenkov EV, Zarembo EV (2005) In vitro regeneration and callogenesis in tissue culture of floral organs of the genus Iris (Iridaceae). Biol Bull 32(2):138–142CrossRefGoogle Scholar
  3. Burchett M, Nash S, Richardson N (1989) Doryanthes excelsa as a commercial crop. Aust Hortic 87(9):36–39Google Scholar
  4. Ellyard RK (1978) In vitro propagation of Anigozanthos manglesii, Anigozanthus flavidus and Macropidia fuliginosa. HortScience 13(6):662–663Google Scholar
  5. Fay MF, Rudall PJ, Sullivan S, Stobart KL, De Bruijn AY, Reeves G, Qamaruz-Zaman F, Hong W-P, Joseph J, Hahn WJ, Conran JG, Chase MW (2000) Phylogenetic studies of asparagales based on four plastid DNA regions. Monocots: systematics and evolution. In: Wilson KL, Morrison DA (eds). Commonwealth Scientific and Industrial Research Organisation (CSIRO) Publishing, Melbourne, Vic., AustraliaGoogle Scholar
  6. Genkov T, Tsoneva P, Ivanova I (1997) Effect of cytokinins on photosynthtic pigments and chlorophyllase activity in in vitro cultures of axillary buds of Dianthus caryophyllus L. J Plant Growth Regul 16:169–172CrossRefGoogle Scholar
  7. Gorst JR (1996) Tissue culture of Australian monocotyledons including the orchids. In: Taji A, Williams R (eds). Tissue culture of Australian plants. University of New England Printery, Armidale, NSW, Australia, pp 56–111Google Scholar
  8. Greisbach RJ (1989) Selection of dwarf Hemerocallis through tissue culture. HortScience 24(6):1027–1028Google Scholar
  9. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ 33:105–119CrossRefGoogle Scholar
  10. Johnson KA (2000) Induction of somatic embryogenesis in Australian monocots. Acta Hortic 520:67–74Google Scholar
  11. Johnson KA, Burchett M (1991) In vitro propagation of Blandfordia grandiflora (Liliaceae). J Hortic Sci 66(4):389–394Google Scholar
  12. Mok MC, Mok DWS (1985) The metabolism of [14C]-thidiazuron in callus tissues of Phaseolus lunatus. Physiol Plant 65:427–432CrossRefGoogle Scholar
  13. Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  14. Murthy BNS, Murch SJ, Savxena PK (1998) Thidiazuron: a potent regulator of in vitro plant morphogenesis. In Vitro Cell Dev Plant 34:267–275Google Scholar
  15. Newman IV (1928) The life history of Doryanthes excelsa: part 1: some ecological and vegetative features and spore production. In: Proceedings of the Linnaean Society of New South Wales, vol 53. pp499–528Google Scholar
  16. Ramsey JL, Galitz DS, Lee CW (2003) Basal medium and sucrose concentration influence regeneration of Easter Lily in ovary culture. HortScience 38(3):404–406Google Scholar
  17. Ricci A, Carra A, Torelli A, Maggiali CA, Vicini P, Zani F, Branca C (2001) Cytokinin-like activity of N’-substituted N-phenylureas. Plant Growth Regul 34:167–172CrossRefGoogle Scholar
  18. Shibli RA, Ajlouni MM (2000) Somatic embryogenesis in the endemic black iris. Plant Cell Tissue Organ 61(1):15–21CrossRefGoogle Scholar
  19. Shimizu K, Nagaike H, Yabuya T, Adachi T (1997) Plant regeneration from suspension culture of Iris germanica. Plant Cell Tissue Organ 50:27–31CrossRefGoogle Scholar
  20. Torres KC (1989) Tissue culture techniques for horticultural crops, edn. AVI-Van Nostrand New York, NY, USAGoogle Scholar
  21. Velcheva M, Faltin Z, Vardi A, Eshdat Y, Perl A (2005) Regeneration of Aloë arborescens via somatic organogenesis from young inflorescences. Plant Cell Tissue Organ 83:293–301CrossRefGoogle Scholar
  22. Visser C, Fletcher RA, Saxena PK (1995) TDZ stimulates expansion and greening in cucumber cotyledons. Physiol Mol Biol Plants 1:21–26Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Adam M. Dimech
    • 1
    • 2
    Email author
  • Rob Cross
    • 1
  • Rebecca Ford
    • 2
  • Paul W. J. Taylor
    • 2
  1. 1.Royal Botanic Gardens MelbourneSouth YarraAustralia
  2. 2.Faculty of Land and Food ResourcesUniversity of MelbourneRoyal ParadeAustralia

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