Plant Cell, Tissue and Organ Culture

, Volume 87, Issue 3, pp 329–331 | Cite as

In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

  • B. DuquenneEmail author
  • T. Eeckhaut
  • S. Werbrouck
  • J. Van Huylenbroeck
Research Note


A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l−1 in combination with 2 mg l−1 α-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l−1 6-τ-τ-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia.


Araceae Callus formation In vitro culture Somatic embryo induction Tubers 





2,4-dichlorophenoxyacetic acid




Murashige and Skoog basal medium


α-naphthaleneacetic acid




  1. Chen F, Kuehnle A (1999) Micropropagation of Spathiphyllum cv. Sensation from in vitro etiolated internode explants. In: XVI International Botanical Congress, St Louis, Book of abstracts, nr 2383Google Scholar
  2. Funnell K (1993). Zantedeschia. In: De Hertogh A, Le Nard M (eds) The physiology of flower bulbs. Elsevier, AmsterdamGoogle Scholar
  3. Gomez L, Valverde R, Arias O, Thorpe T (1992). Regeneration of Xanthosoma sagittifolium by somatic embryogenesis. Agron Costarric 16:219–223Google Scholar
  4. Hamidah M, Karim A, Debergh P (1997). Somatic embryogenesis and plant regeneration in Anthurium scherzerianum. PCTOC 48:189–193CrossRefGoogle Scholar
  5. Matsumoto T, Webb D, Kuehnle A (1996). Histology and origin of somatic embryos derived rom Anthurium andreanum Linden ex Andre lamina. J Am Soc Hort Sci 121:404–407Google Scholar
  6. Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  7. Werbrouck S, Eeckhaut T, Debergh P (2000). Induction and conversion of somatic embryogenesis on the anther filament of Spathiphyllum Schott. Acta Hortic 520:263–269Google Scholar
  8. Wu A, Zhu B, Li Q (1996). Studies on somatic embryogenesis of Pinellia pedatisecta Schott. Acta Agron. Sin 22:66–71Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • B. Duquenne
    • 1
    • 2
    Email author
  • T. Eeckhaut
    • 1
  • S. Werbrouck
    • 2
  • J. Van Huylenbroeck
    • 1
  1. 1.Plant Unit – Applied Genetics and BreedingInstitute for Agricultural and Fisheries ResearchMelleBelgium
  2. 2.Department of BiotechnologyHogeschool GentGentBelgium

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