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Plant Cell, Tissue and Organ Culture

, Volume 87, Issue 3, pp 329–331 | Cite as

In vitro somatic embryogenesis and plant regeneration in Zantedeschia hybrids

  • B. DuquenneEmail author
  • T. Eeckhaut
  • S. Werbrouck
  • J. Van Huylenbroeck
Research Note

Abstract

A method for regeneration of plants from tuber explants of a Zantedeschia hybrid via somatic embryogenesis was developed. In vitro cultures were initiated starting from both anthers and tubers. Somatic embryogenesis was only achieved from tuber explants. 6-Benzyladenine (BA) at 0.6 or 2 mg l−1 in combination with 2 mg l−1 α-naphthaleneacetic acid (NAA) yielded the highest number of embryos per explant. The somatic embryos converted into plantlets on Murashige and Skoog basal medium supplemented with vitamins, micro- and macronutrients, 1 mg l−1 6-τ-τ-(dimethylallylamino)-purine (2iP), 3% sucrose and 0.7% agar. This is the first report on induction of somatic embryogenesis in Zantedeschia.

Keywords

Araceae Callus formation In vitro culture Somatic embryo induction Tubers 

Abbreviations

BA

6-benzyladenine

2,4-D

2,4-dichlorophenoxyacetic acid

2iP

6-τ-τ-(dimethylallylamino)-purine

MS

Murashige and Skoog basal medium

NAA

α-naphthaleneacetic acid

TDZ

Thidiazuron

References

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • B. Duquenne
    • 1
    • 2
    Email author
  • T. Eeckhaut
    • 1
  • S. Werbrouck
    • 2
  • J. Van Huylenbroeck
    • 1
  1. 1.Plant Unit – Applied Genetics and BreedingInstitute for Agricultural and Fisheries ResearchMelleBelgium
  2. 2.Department of BiotechnologyHogeschool GentGentBelgium

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