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Plant Cell, Tissue and Organ Culture

, Volume 87, Issue 3, pp 255–262 | Cite as

Dehydration-induced alterations in growth and osmotic potential of callus from six tepary bean lines varying in drought resistance

  • Mohamed F. Mohamed
  • Azza A. Tawfik
Original Paper

Abstract

Growth and osmotic potential of calli induced from leaf- and root-derived tissues of six tepary bean lines (Phaseolus acutifolius) varying in drought resistance were assessed in vitro after polyethylene glycol-induced (10%, PEG-10,000) dehydration. Calli of resistant teparies were characterized by low initial osmotic potential (ψs) and relative growth rate (RGR) on medium lacking PEG (−0.30 MPa). However, calli of both resistant and sensitive lines were similar in dry matter percent (DM). Presence of PEG in the medium (−0.58 MPa) elevated DM in all teparies except one resistant line. Both leaf- and root-derived calli of sensitive teparies exhibited osmotic adjustment (OA) but reduced RGR that remained after rehydration in one line. We concluded that preexisting force of low cellular ψs rather than induced OA plays an important role in buffering adverse effects of dehydration and conditioning drought resistance of tepary beans. This information may aid Phaseolus breeders in screening for drought resistance among large number of accessions.

Keywords

Germplasm In vitro Osmotic adjustment PEG Phaseolus acutifolius Relative growth rate 

Notes

Acknowledgement

The authors are grateful to Prof. Dr. E. D. Earle (Cornell University, Ithaca, New York) for the critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of Horticulture, Faculty of AgricultureAssiut UniversityAssiutEgypt

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