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Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae)

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Abstract

We tested the organogenetic capacity of floral buds of Mammillaria albicoma Böed. (Cactaceae). Buds were incubated on solid MS medium supplemented with 0.1 mg l−1 α-naphthaleneacetic acid (NAA) and 5.0 mg l−1 6-benzylaminopurine (BA). Callus growth was observed from the cut explant base and from within the perianth. These calli during subsequent subcultures to the same medium gave rise to adventitious shoots. Shoots formed also directly from the perianth, as confirmed by observations in the light microscope and scanning electron microscope (SEM). On transfer to a fresh medium, the shoots produced proliferating cultures. This is the first report of regeneration of cactus shoots from floral explants.

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Abbreviations

BA:

6-benzylaminopurine

MS:

Murashige and Skoog

NAA:

α-naphthaleneacetic acid

PPFD:

photosynthetic photon flux density

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Acknowledgement

We thank Dr. David Giblin for linguistic advice.

Author information

Correspondence to Tomasz P. Wyka.

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Wyka, T.P., Hamerska, M. & Wróblewska, M. Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae) . Plant Cell Tiss Organ Cult 87, 27–32 (2006). https://doi.org/10.1007/s11240-006-9128-9

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Keywords

  • Cactaceae
  • Floral explants
  • Mammillaria albicoma
  • Organogenesis