Efficient Somatic Embryogenesis in Alstroemeria
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In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 μM thidiazuron and 0.5 μM indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 μM 2,4-dichlorophenoxy acetic acid and 2.2 μM benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 μM picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 μM BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally.
KeywordsAlstroemeria Compact embryogenic callus Friable embryogenic callus Regeneration Somatic embryos
2,4-dichlorophenoxy acetic acid
Compact embryogenic callus
Callus induction medium
Callus proliferation medium
Friable embryogenic callus
Murashige and Skoog (1962) medium
Schenk and Hildebrandt (1972) medium
Shoot induction medium
N-phenyl-N′-1,2,3-thidiazo-5-yl urea or thidiazuron
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We thank Royal van Zanten (the Netherlands) who kindly provided in vitro plants of the genotype VV024. We thank Dirkjan Huigen and Bert Essenstam for taking care of the plants in the greenhouse. This research was partly financed by the Ministry of Education, Republic of Korea.
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