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Plant Cell, Tissue and Organ Culture

, Volume 86, Issue 2, pp 233–238 | Cite as

Efficient Somatic Embryogenesis in Alstroemeria

  • J. B. Kim
  • C. J. J. M. RaemakersEmail author
  • E. Jacobsen
  • R. G. F. Visser
Original paper

Abstract

In Alstroemeria high frequencies of compact embryogenic callus (CEC) induction (40%) and friable embryogenic callus (FEC) induction (15%) were obtained from nodes with axil tissue cultured first on a Murashige and Skoog (MS) medium supplemented with 10 μM thidiazuron and 0.5 μM indole-3-butyric acid and after that on a Schenk and Hildebrandt (SH) medium supplemented with 9.1 μM 2,4-dichlorophenoxy acetic acid and 2.2 μM benzylaminopurine (BA). Both types of callus were maintained on modified MS medium supplemented with 20.8 μM picloram. CEC and FEC formed somatic embryos and subsequently plants when transferred to MS medium supplemented with 2.2 μM BA. Plants were produced after 12 weeks (CEC) or after 16 weeks (FEC) of culture. Regenerated plants were established in the greenhouse and flowered normally.

Keywords

Alstroemeria Compact embryogenic callus Friable embryogenic callus Regeneration Somatic embryos 

Abbreviations

2,4-d

2,4-dichlorophenoxy acetic acid

BA

Benzylaminopurine

CEC

Compact embryogenic callus

CIM

Callus induction medium

CPM

Callus proliferation medium

FEC

Friable embryogenic callus

IBA

Indole-3-butyric acid

MES

2-(N-morpholino)ethanesulfonic acid

MS

Murashige and Skoog (1962) medium

NAA

α-naphthaleneacetic acid

NEC

Nonembryogenic callus

PM

Propagation medium

SE

Somatic embryo

SH

Schenk and Hildebrandt (1972) medium

SIM

Shoot induction medium

TDZ

N-phenyl-N′-1,2,3-thidiazo-5-yl urea or thidiazuron

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Notes

Acknowledgments

We thank Royal van Zanten (the Netherlands) who kindly provided in vitro plants of the genotype VV024. We thank Dirkjan Huigen and Bert Essenstam for taking care of the plants in the greenhouse. This research was partly financed by the Ministry of Education, Republic of Korea.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • J. B. Kim
    • 1
    • 2
  • C. J. J. M. Raemakers
    • 1
    Email author
  • E. Jacobsen
    • 1
  • R. G. F. Visser
    • 1
  1. 1.Laboratory of Plant Breeding, The Graduate School of Experimental Plant SciencesWageningen UniversityWageningenThe Netherlands
  2. 2.Central Research Institute Neobio Co., LtdKyong-kiKorea

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